Transcription factor cellular promoter 2 is required for upstream binding protein 1 -mediated angiogenesis

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY Gene Expression Patterns Pub Date : 2023-06-01 DOI:10.1016/j.gep.2023.119308

Yanyan Ren , YaneYang , Qingbo Lu , Qiang Wang , Gentao Lu , Yanli Wei , Jiaqi Zhou

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Abstract

Objective

Angiogenesis is a key process of repairing tissue damage, and it is regulated by the delicate balance between anti-angiogenesis factors. In the present study, we investigate whether transcription factor cellular promoter 2 (TFCP2) is required for upstream binding protein 1 (UBP1)-mediated angiogenesis.

Methods

Levels of UBP1 and TFCP2 in human umbilical vein endothelial cells (HUVECs) are detected by quantitative polymerase chain reaction (q-PCR) and Western blotting (WB). Effects of UBP1 on angiogenesis and migration are detected by tube-like network formation on matrigel assay and scratch assay. The interaction between UBP1 and TFCP2 is predicted and verified by STRING and Co-immunoprecipitation (Co-IP).

Results

Firstly, the UBP1 expression level was up-regulated in the stimuli of vascular endothelial growth factor (VEGF) in HUVECs, and the knockdown of UBP1 inhibited angiogenesis and migration of HUVECs. Then, UBP1 interacted with TFCP2. Besides, the TFCP2 expression level was up-regulated in VEGF-stimulated HUVECs. Furthermore, knockdown of TFCP2 inhibited angiogenesis and migration in VEGF-stimulated HUVECs, and down-regulation of UBP1 enhanced the inhibition.

Conclusion

TFCP2 also plays a key role in UBP1 mediated angiogenesis of HUVECs stimulated by VEGF. These findings will provide a new theoretical basis for the treatment of angiogenic diseases.

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转录因子细胞启动子2是上游结合蛋白1介导的血管生成所必需的

目的血管生成是组织损伤修复的关键过程，受抗血管生成因子之间的微妙平衡调控。在本研究中，我们研究了上游结合蛋白1 (UBP1)介导的血管生成是否需要转录因子细胞启动子2 (TFCP2)。方法采用定量聚合酶链式反应(q-PCR)和Western blotting (WB)检测人脐静脉内皮细胞(HUVECs)中UBP1和TFCP2的表达水平。在基质实验和划痕实验中，通过管状网络的形成检测UBP1对血管生成和迁移的影响。通过STRING和Co-immunoprecipitation (Co-IP)预测并验证了UBP1和TFCP2之间的相互作用。结果首先，在血管内皮生长因子(VEGF)刺激下，UBP1的表达水平上调，UBP1的下调抑制了HUVECs的血管生成和迁移。然后，UBP1与TFCP2相互作用。此外，在vegf刺激的HUVECs中，TFCP2表达水平上调。此外，在vegf刺激的HUVECs中，TFCP2的下调抑制了血管生成和迁移，UBP1的下调增强了抑制作用。结论tfcp2在UBP1介导的VEGF刺激HUVECs血管生成中也起关键作用。这些发现将为血管生成疾病的治疗提供新的理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene Expression Patterns 生物-发育生物学

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

35 days

期刊介绍： Gene Expression Patterns is devoted to the rapid publication of high quality studies of gene expression in development. Studies using cell culture are also suitable if clearly relevant to development, e.g., analysis of key regulatory genes or of gene sets in the maintenance or differentiation of stem cells. Key areas of interest include: -In-situ studies such as expression patterns of important or interesting genes at all levels, including transcription and protein expression -Temporal studies of large gene sets during development -Transgenic studies to study cell lineage in tissue formation

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