Proteomics analysis of methionine enkephalin upregulated macrophages against infection by the influenza-A virus.

IF 2.1 3区 生物学 Q3 BIOCHEMICAL RESEARCH METHODS Proteome Science Pub Date : 2023-04-11 DOI:10.1186/s12953-023-00205-w
Wenrui Fu, Zifeng Xie, Mei Bai, Zhen Zhang, Yuanlong Zhao, Jing Tian
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引用次数: 1

Abstract

Macrophages have a vital role in phagocytosis and antiviral effect against invading influenza viruses. Previously, we found that methionine enkephalin (MENK) inhibited influenza virus infection by upregulating the "antiviral state" of macrophages. To investigate the immunoregulatory mechanism of action of MENK on macrophages, we employed proteomic analysis to identify differentially expressed proteins (DEPs) between macrophages infected with the influenza-A virus and cells infected with the influenza-A virus after pretreatment with MENK. A total of 215 DEPs were identified: 164 proteins had upregulated expression and 51 proteins had downregulated expression. Proteomics analysis showed that DEPs were highly enriched in "cytokine-cytokine receptor interaction", "phagosome", and "complement and coagulation cascades pathway". Proteomics analysis revealed that MENK could be an immune modulator or prophylactic for the prevention and treatment of influenza. MENK promoted the polarization of M1 macrophages, activated inflammatory responses, and enhanced phagocytosis and killing function by upregulating opsonizing receptors.

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蛋氨酸脑啡肽上调巨噬细胞抗流感a型病毒感染的蛋白质组学分析。
巨噬细胞对入侵的流感病毒具有重要的吞噬作用和抗病毒作用。先前,我们发现蛋氨酸脑啡肽(MENK)通过上调巨噬细胞的“抗病毒状态”来抑制流感病毒感染。为了研究MENK对巨噬细胞的免疫调节机制,我们采用蛋白质组学分析方法鉴定了经MENK预处理后感染流感a- a病毒的巨噬细胞与感染流感a- a病毒的细胞之间的差异表达蛋白(DEPs)。共鉴定出215个DEPs,其中164个蛋白表达上调,51个蛋白表达下调。蛋白质组学分析显示,DEPs在“细胞因子-细胞因子受体相互作用”、“吞噬体”和“补体与凝血级联通路”中高度富集。蛋白质组学分析表明,MENK可能是预防和治疗流感的免疫调节剂或预防剂。MENK通过上调调理受体,促进M1巨噬细胞极化,激活炎症反应,增强吞噬和杀伤功能。
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来源期刊
Proteome Science
Proteome Science 生物-生化研究方法
CiteScore
2.90
自引率
0.00%
发文量
17
审稿时长
4.5 months
期刊介绍: Proteome Science is an open access journal publishing research in the area of systems studies. Proteome Science considers manuscripts based on all aspects of functional and structural proteomics, genomics, metabolomics, systems analysis and metabiome analysis. It encourages the submissions of studies that use large-scale or systems analysis of biomolecules in a cellular, organismal and/or environmental context. Studies that describe novel biological or clinical insights as well as methods-focused studies that describe novel methods for the large-scale study of any and all biomolecules in cells and tissues, such as mass spectrometry, protein and nucleic acid microarrays, genomics, next-generation sequencing and computational algorithms and methods are all within the scope of Proteome Science, as are electron topography, structural methods, proteogenomics, chemical proteomics, stem cell proteomics, organelle proteomics, plant and microbial proteomics. In spite of its name, Proteome Science considers all aspects of large-scale and systems studies because ultimately any mechanism that results in genomic and metabolomic changes will affect or be affected by the proteome. To reflect this intrinsic relationship of biological systems, Proteome Science will consider all such articles.
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