Role of Immunohistochemistry in the Differential Diagnosis of Pediatric Renal Tumors: Expression of Cyclin D1, Beta-Catenin , PDGFR-Alpha, and PTEN.

IF 1.1 Q4 PATHOLOGY Turkish Journal of Pathology Pub Date : 2022-01-01 DOI:10.5146/tjpath.2022.01568

Nuray Kepil, Şebnem Batur, Zeynep Ecem Kain, Gamze Özcan, Şenol Emre, Rahşan Özcan, Tülin Tiraje Celkan, Nil Çomunoğlu

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Abstract

Objective: Pediatric renal tumors overlap histomorphologically and may cause misdiagnosis. We aimed to determine the role of immunohistochemical staining of Cyclin D1, PTEN, beta-catenin and PDGFR-alpha on pediatric renal tumors.

Material and method: Thirty-six cases of 8 different tumors were included in the study. Four blocks of paraffin tissue microarray were constructed. Cyclin D1, PTEN, beta-catenin and PDGFR-alpha were used in all cases. Staining intensity and extent were graded.

Results: All cases of clear cell sarcoma (CCS) and epithelial components of Wilms tumor (WT) showed immunopositivity for Cyclin D1 but blastemal and stromal components of WT were negative. All cases of CCS and most cases of WT consisting of blastemal and stromal components demonstrated loss of expression with PTEN.

Conclusion: Cyclin D1 is not a specific immunohistochemical marker due to its strong and diffuse positivity in CCS cases. It may be useful to differentiate CCS from blastemal and stromal components of WT. Other markers except cyclin D1 do not have a role in the differential diagnosis.

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免疫组织化学在小儿肾肿瘤鉴别诊断中的作用:细胞周期蛋白D1、β -连环蛋白、pdgfr - α和PTEN的表达

目的:探讨小儿肾肿瘤的组织学重叠及易引起误诊的原因。我们的目的是确定免疫组化染色的Cyclin D1, PTEN， β -连环蛋白和pdgfr - α在儿童肾肿瘤中的作用。材料与方法:选取8种不同肿瘤36例进行研究。构建了4块石蜡组织芯片。所有病例均使用Cyclin D1、PTEN、β -连环蛋白和pdgfr - α。对染色强度和程度进行分级。结果:所有透明细胞肉瘤(CCS)和Wilms肿瘤(WT)上皮成分均显示Cyclin D1免疫阳性，而WT的胚质成分和基质成分均为阴性。所有的CCS病例和大多数由胚质和基质组成的WT病例都表现出PTEN的表达缺失。结论:Cyclin D1在CCS病例中呈强弥漫性阳性，不是特异性免疫组织化学标志物。这可能有助于将CCS与WT的胚质和基质成分区分开来。除了细胞周期蛋白D1外，其他标志物在鉴别诊断中没有作用。

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