Template-Based Porous Hydrogel Microparticles as Carriers for Therapeutic Proteins

IF 3.8 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY ACS Bio & Med Chem Au Pub Date : 2023-03-10 DOI:10.1021/acsbiomedchemau.3c00001
Philippe Delbreil, Xavier Banquy and Davide Brambilla*, 
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引用次数: 2

Abstract

Hydrogels have been extensively researched for over 60 years for their limitless applications in biomedical research. In this study, porous hydrogel microparticles (PHMPs) made of poly(ethylene glycol) diacrylamide were investigated for their potential as a delivery platform for therapeutic proteins. These particles are made using hard calcium carbonate (CaCO3) templates, which can easily be dissolved under acidic conditions. After optimization of the synthesis processes, both CaCO3 templates and PHMPs were characterized using a wide range of techniques. Then, using an array of proteins with different physicochemical properties, the encapsulation efficiency of proteins in PHMPs was evaluated under different conditions. Strategies to enhance protein encapsulation via modulation of particle surface charge to increase electrostatic interactions and conjugation using EDC/NHS chemistry were also investigated. Conjugation of bovine serum albumin to PHMPs showed increased encapsulation and diminished release over time, highlighting the potential of PHMPs as a versatile delivery platform for therapeutic proteins such as enzymes or antibodies.

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基于模板的多孔水凝胶微颗粒作为治疗性蛋白的载体
水凝胶因其在生物医学研究中的无限应用而被广泛研究了60多年。在本研究中,研究了由聚乙二醇二丙烯酰胺制成的多孔水凝胶微粒(PHMP)作为治疗蛋白质递送平台的潜力。这些颗粒是用硬质碳酸钙(CaCO3)模板制成的,在酸性条件下很容易溶解。在优化合成工艺后,使用各种技术对CaCO3模板和PHMP进行了表征。然后,使用具有不同物理化学性质的蛋白质阵列,在不同条件下评估蛋白质在PHMP中的包封效率。还研究了通过调节颗粒表面电荷以增加静电相互作用和使用EDC/NHS化学结合来增强蛋白质包封的策略。牛血清白蛋白与PHMP的偶联显示出随时间的推移包封增加和释放减少,突出了PHMP作为治疗蛋白质(如酶或抗体)的多功能递送平台的潜力。
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来源期刊
ACS Bio & Med Chem Au
ACS Bio & Med Chem Au 药物、生物、化学-
CiteScore
4.10
自引率
0.00%
发文量
0
期刊介绍: ACS Bio & Med Chem Au is a broad scope open access journal which publishes short letters comprehensive articles reviews and perspectives in all aspects of biological and medicinal chemistry. Studies providing fundamental insights or describing novel syntheses as well as clinical or other applications-based work are welcomed.This broad scope includes experimental and theoretical studies on the chemical physical mechanistic and/or structural basis of biological or cell function in all domains of life. It encompasses the fields of chemical biology synthetic biology disease biology cell biology agriculture and food natural products research nucleic acid biology neuroscience structural biology and biophysics.The journal publishes studies that pertain to a broad range of medicinal chemistry including compound design and optimization biological evaluation molecular mechanistic understanding of drug delivery and drug delivery systems imaging agents and pharmacology and translational science of both small and large bioactive molecules. Novel computational cheminformatics and structural studies for the identification (or structure-activity relationship analysis) of bioactive molecules ligands and their targets are also welcome. The journal will consider computational studies applying established computational methods but only in combination with novel and original experimental data (e.g. in cases where new compounds have been designed and tested).Also included in the scope of the journal are articles relating to infectious diseases research on pathogens host-pathogen interactions therapeutics diagnostics vaccines drug-delivery systems and other biomedical technology development pertaining to infectious diseases.
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