Archaeal pseudomurein and bacterial murein cell wall biosynthesis share a common evolutionary ancestry.

Bishwa P Subedi, William F Martin, Vincenzo Carbone, Eduardus C Duin, Bryan Cronin, Julia Sauter, Linley R Schofield, Andrew J Sutherland-Smith, Ron S Ronimus
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引用次数: 9

Abstract

Bacteria near-universally contain a cell wall sacculus of murein (peptidoglycan), the synthesis of which has been intensively studied for over 50 years. In striking contrast, archaeal species possess a variety of other cell wall types, none of them closely resembling murein. Interestingly though, one type of archaeal cell wall termed pseudomurein found in the methanogen orders Methanobacteriales and Methanopyrales is a structural analogue of murein in that it contains a glycan backbone that is cross-linked by a L-amino acid peptide. Here, we present taxonomic distribution, gene cluster and phylogenetic analyses that confirm orthologues of 13 bacterial murein biosynthesis enzymes in pseudomurein-containing methanogens, most of which are distantly related to their bacterial counterparts. We also present the first structure of an archaeal pseudomurein peptide ligase from Methanothermus fervidus DSM1088 (Mfer336) to a resolution of 2.5 Å and show that it possesses a similar overall tertiary three domain structure to bacterial MurC and MurD type murein peptide ligases. Taken together the data strongly indicate that murein and pseudomurein biosynthetic pathways share a common evolutionary history.

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古细菌假尿素和细菌鼠细胞壁生物合成具有共同的进化祖先。
几乎所有的细菌都含有一个由肽聚糖组成的细胞壁小囊,其合成已经被深入研究了50多年。与之形成鲜明对比的是,古细菌物种拥有多种其他细胞壁类型,没有一种与小鼠细胞壁非常相似。有趣的是,在产甲烷菌目甲烷杆菌目和甲烷菌目中发现的一种被称为假尿素的古细菌细胞壁是一种类似于鼠尿素的结构,因为它含有一个由l -氨基酸肽交联的聚糖骨架。本文通过对含假尿素甲烷菌中13种细菌小鼠生物合成酶的分类分布、基因聚类和系统发育分析,证实了它们的同源物,其中大多数与细菌的同源物有远亲关系。我们还报道了来自Methanothermus fervidus DSM1088 (Mfer336)的古细菌假尿蛋白肽连接酶的第一个结构,分辨率为2.5 Å,并表明它具有与细菌MurC和MurD型尿蛋白肽连接酶相似的三级结构域结构。综上所述,这些数据有力地表明,鼠尿素和假尿素的生物合成途径具有共同的进化史。
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3.30
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审稿时长
15 weeks
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