Characterization of a Diverse Collection of Salmonella Phages Isolated from Tennessee Wastewater.

PHAGE (New Rochelle, N.Y.) Pub Date : 2023-06-01 DOI:10.1089/phage.2023.0004

Daniel W Bryan, Lauren K Hudson, Jia Wang, Thomas G Denes

{"title":"Characterization of a Diverse Collection of Salmonella Phages Isolated from Tennessee Wastewater.","authors":"Daniel W Bryan, Lauren K Hudson, Jia Wang, Thomas G Denes","doi":"10.1089/phage.2023.0004","DOIUrl":null,"url":null,"abstract":"Background: Salmonella enterica is one of the most prevalent bacterial foodborne pathogens. Salmonella phages are currently used in biocontrol applications and have potential for use as therapeutics.Materials and methods: Phages were enriched and purified from a diversity of Salmonella host isolates. Morphology was determined with transmission electron microscopy, host ranges were characterized using an efficiency of plaquing assay, and comparative genomic analysis was performed to determine taxonomy.Results: Ten phages were isolated and characterized. Phages showed activity against 23 out of the 24 Salmonella serovars evaluated. Two phages also showed activity against Escherichia coli strain B. Phages belonged to five different genera (Ithacavirus, Gelderlandvirus, Kuttervirus, Tlsvirus, and Epseptimavirus), two established species, and eight novel species.Conclusions: The phages described here further demonstrate the diversity of S. enterica phages present in wastewater effluent. This work contributes a collection of characterized phages from eastern Tennessee that may be of use in future phage-based applications targeting S. enterica.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 2","pages":"90-98"},"PeriodicalIF":0.0000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282790/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"PHAGE (New Rochelle, N.Y.)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/phage.2023.0004","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Salmonella enterica is one of the most prevalent bacterial foodborne pathogens. Salmonella phages are currently used in biocontrol applications and have potential for use as therapeutics.

Materials and methods: Phages were enriched and purified from a diversity of Salmonella host isolates. Morphology was determined with transmission electron microscopy, host ranges were characterized using an efficiency of plaquing assay, and comparative genomic analysis was performed to determine taxonomy.

Results: Ten phages were isolated and characterized. Phages showed activity against 23 out of the 24 Salmonella serovars evaluated. Two phages also showed activity against Escherichia coli strain B. Phages belonged to five different genera (Ithacavirus, Gelderlandvirus, Kuttervirus, Tlsvirus, and Epseptimavirus), two established species, and eight novel species.

Conclusions: The phages described here further demonstrate the diversity of S. enterica phages present in wastewater effluent. This work contributes a collection of characterized phages from eastern Tennessee that may be of use in future phage-based applications targeting S. enterica.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

从田纳西州废水中分离的多种噬菌体沙门氏菌的特性。

背景:肠沙门氏菌是最常见的细菌性食源性致病菌之一。沙门氏菌噬菌体目前用于生物防治应用，并有潜力用作治疗药物。材料和方法:从多种沙门氏菌宿主分离物中富集和纯化噬菌体。通过透射电子显微镜观察其形态，利用菌斑效率测定对宿主范围进行表征，并进行比较基因组分析以确定其分类。结果:分离得到10个噬菌体。噬菌体对24种沙门氏菌血清型中的23种有活性。两种噬菌体也显示出对大肠杆菌b株的活性。噬菌体属于5个不同的属(Ithacavirus, Gelderlandvirus, Kuttervirus, Tlsvirus和Epseptimavirus)， 2个已建立的种和8个新种。结论:本文描述的噬菌体进一步证明了污水中存在的肠球菌噬菌体的多样性。这项工作贡献了来自田纳西州东部的特征噬菌体的集合，可能在未来的噬菌体为基础的应用靶向肠球菌使用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

PHAGE (New Rochelle, N.Y.)

自引率

0.00%

发文量