Influence of Cell Type on the Efficacy of Plasmonic Photothermal Therapy

IF 4.8 Q2 NANOSCIENCE & NANOTECHNOLOGY ACS Nanoscience Au Pub Date : 2022-07-27 DOI:10.1021/acsnanoscienceau.2c00023
Helena Villuendas, Clara Vilches* and Romain Quidant*, 
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引用次数: 2

Abstract

In plasmonic photothermal therapy (PPTT), illuminated gold nanoparticles are locally heated to produce selective damage in cells. While PPTT is expected to strongly depend on the cell line, available data are sparse and critical parameters remain unclear. To elucidate this pivotal aspect, we present a systematic study of diseased and nondiseased cells from different tissues to evaluate cytotoxicity, uptake of gold nanorods (AuNRs), and viability after PPTT. We identified differences in uptake and toxicity between cell types, linking AuNR concentrations to toxicity. Furthermore, the cell death mechanism is shown to depend on the intensity of the irradiated light and hence the temperature increase. Importantly, the data also underline the need to monitor cell death at different time points. Our work contributes to the definition of systematic protocols with appropriate controls to fully comprehend the effects of PPTT and build meaningful and reproducible data sets, key to translate PPTT to clinical settings.

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细胞类型对等离子体光热治疗效果的影响
在等离子体光热疗法(PPTT)中,被照亮的金纳米粒子被局部加热以在细胞中产生选择性损伤。虽然预计PPTT在很大程度上依赖于细胞系,但可用的数据很少,关键参数仍不清楚。为了阐明这一关键方面,我们对来自不同组织的患病和非患病细胞进行了系统研究,以评估PPTT后的细胞毒性、金纳米棒(aunr)的摄取和活力。我们确定了细胞类型之间摄取和毒性的差异,将AuNR浓度与毒性联系起来。此外,细胞死亡机制显示取决于辐照光的强度,因此温度升高。重要的是,这些数据还强调了在不同时间点监测细胞死亡的必要性。我们的工作有助于定义具有适当控制的系统方案,以充分理解PPTT的影响,并建立有意义和可重复的数据集,这是将PPTT转化为临床设置的关键。
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来源期刊
ACS Nanoscience Au
ACS Nanoscience Au 材料科学、纳米科学-
CiteScore
4.20
自引率
0.00%
发文量
0
期刊介绍: ACS Nanoscience Au is an open access journal that publishes original fundamental and applied research on nanoscience and nanotechnology research at the interfaces of chemistry biology medicine materials science physics and engineering.The journal publishes short letters comprehensive articles reviews and perspectives on all aspects of nanoscience and nanotechnology:synthesis assembly characterization theory modeling and simulation of nanostructures nanomaterials and nanoscale devicesdesign fabrication and applications of organic inorganic polymer hybrid and biological nanostructuresexperimental and theoretical studies of nanoscale chemical physical and biological phenomenamethods and tools for nanoscience and nanotechnologyself- and directed-assemblyzero- one- and two-dimensional materialsnanostructures and nano-engineered devices with advanced performancenanobiotechnologynanomedicine and nanotoxicologyACS Nanoscience Au also publishes original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials engineering physics bioscience and chemistry into important applications of nanomaterials.
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