Characterization of Lactic Acid Bacteria from Fermented Fish (pla-paeng-daeng) and Their Cholesterol-lowering and Immunomodulatory Effects.

IF 2 4区环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Microbes and Environments Pub Date : 2023-01-01 DOI:10.1264/jsme2.ME22044

Engkarat Kingkaew, Hiroshi Konno, Yoshihito Hosaka, Wongsakorn Phongsopitanun, Somboon Tanasupawat

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引用次数: 2

Abstract

The cholesterol-lowering and immunomodulatory effects and probiotic properties of 25 lactic acid bacteria (LAB) isolated from fermented fish (pla-paeng-daeng) in Thailand were examined in the present study. Based on their phenotypic and genetic characteristics, LAB were identified as Lactiplantibacillus pentosus (Group I, 6 isolates), Lactiplantibacillus argentoratensis (Group II, 1 isolate), Limosilactobacillus fermentum (Group III, 2 isolates), Companilactobacillus pabuli (Group IV, 4 isolates), Companilactobacillus farciminis (Group V, 5 isolates), Companilactobacillus futsaii (Group VI, 6 isolates), and Enterococcus lactis (Group VII, 1 isolate). Lactiplantibacillus pentosus PD3-1 and PD9-2 and Enterococcus lactis PD3-2 exhibited bile salt hydrolase (BSH) activities. The percentage of cholesterol assimilated by all isolates ranged between 21.40 and 54.07%. Bile salt hydrolase-producing isolates tolerated acidic and bile conditions and possessed adhesion properties. They also exerted immunomodulatory effects that affected the production of interleukin-12 (IL-12), interferon-γ (IFN-γ), human β-defensin-2 (hBD-2), and nitric oxide (NO). These isolates meet standard probiotic requirements and exert beneficial effects.

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发酵鱼乳酸菌的特性及其降胆固醇和免疫调节作用。

研究了从泰国发酵鱼(pla-paeng-daeng)中分离的25株乳酸菌(LAB)的降胆固醇和免疫调节作用及其益生菌特性。根据表型和遗传特征鉴定乳酸菌为戊式乳酸菌(第1群，6株)、阿根廷乳酸菌(第2群，1株)、发酵乳酸杆菌(第3群，2株)、食腐乳酸菌(第4群，4株)、farciminis乳酸菌(第5群，5株)、富塞乳酸菌(第6群，6株)和乳酸肠球菌(第7群，1株)。戊乳杆菌PD3-1、PD9-2和乳酸肠球菌PD3-2均有胆盐水解酶活性。所有菌株的胆固醇同化率在21.40 ~ 54.07%之间。产生胆汁盐水解酶的分离株耐受酸性和胆汁条件，并具有粘附特性。它们还发挥免疫调节作用，影响白细胞介素-12 (IL-12)、干扰素-γ (IFN-γ)、人β-防御素-2 (hBD-2)和一氧化氮(NO)的产生。这些分离物符合标准益生菌要求，并发挥有益作用。

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来源期刊

Microbes and Environments 生物-生物工程与应用微生物

CiteScore

4.10

自引率

13.60%

发文量

审稿时长

3 months

期刊介绍： Microbial ecology in natural and engineered environments; Microbial degradation of xenobiotic compounds; Microbial processes in biogeochemical cycles; Microbial interactions and signaling with animals and plants; Interactions among microorganisms; Microorganisms related to public health; Phylogenetic and functional diversity of microbial communities; Genomics, metagenomics, and bioinformatics for microbiology; Application of microorganisms to agriculture, fishery, and industry; Molecular biology and biochemistry related to environmental microbiology; Methodology in general and environmental microbiology; Interdisciplinary research areas for microbial ecology (e.g., Astrobiology, and Origins of Life); Taxonomic description of novel microorganisms with ecological perspective; Physiology and metabolisms of microorganisms; Evolution of genes and microorganisms; Genome report of microorganisms with ecological perspective.