Virulence comparison of 4 porcine circovirus type 2 (PCV-2) genotypes: 2a, 2b, 2d, and 2e with a single infection and co-infection with PCV-2 and porcine reproductive and respiratory syndrome virus (PRRSV).

IF 1.1 4区 农林科学 Q2 Veterinary Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2023-01-01
Jeongmin Suh, Taehwan Oh, Chanhee Chae
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Abstract

The objective of this study was to compare the virulence of 4 porcine circovirus type 2 (PCV-2) genotypes (2a, 2b, 2d, and 2e) in pigs singly infected with 1 of these 4 PCV-2 genotypes and pigs dually infected with a combination of 1 of the 4 PCV-2 genotypes and porcine reproductive and respiratory syndrome virus (PRRSV). Virulence was determined based on levels of PCV-2 loads in the blood and lymph nodes and the severity of lymphoid lesion. Within the singly infected groups, PCV-2a, PCV-2b, and PCV-2d resulted in a similar virulence to each other and all were more virulent than the PCV-2e groups. Within the dually infected groups, the combination of PCV-2d and PRRSV was more virulent than the other 3 PCV-2 genotypes (2a, 2b, and 2e), each in combination with PRRSV. Both PCV-2a+PRRSV and PCV-2b+PRRSV were more virulent than PCV-2e+PRRSV in dually infected pigs. This increased virulence of PCV-2d compared to the other 3 PCV-2 genotypes (2a, 2b, and 2e) may be attributed to an extra amino acid (lysine residue) found within open reading frame 2 (ORF2) of PCV-2d. In contrast, extra amino acids in ORF2 may decrease the virulence of PCV-2e when compared to the other 3 PCV-2 genotypes (2a, 2b, and 2d). The results of this study demonstrated that PCV-2d was the most virulent PCV-2 genotype in pigs co-infected with PRRSV. The results also suggest that genetic differences in the ORF2 of PCV-2 may affect the virulence of PCV-2 genotypes.

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猪圆环病毒2型(PCV-2)基因型2a、2b、2d和2e与PCV-2和猪繁殖与呼吸综合征病毒(PRRSV)单感染和共感染的毒力比较
本研究的目的是比较4种猪圆环病毒2型(PCV-2)基因型(2a、2b、2d和2e)在单独感染这4种PCV-2基因型中的一种的猪和同时感染4种PCV-2基因型中的一种与猪繁殖与呼吸综合征病毒(PRRSV)的猪中的毒力。毒力是根据血液和淋巴结中PCV-2的负荷水平以及淋巴病变的严重程度来确定的。在单感染组中,PCV-2a、PCV-2b和PCV-2d的毒力相似,均比PCV-2e组的毒力更强。在双重感染组中,PCV-2d和PRRSV联合感染比其他3种PCV-2基因型(2a、2b和2e)分别与PRRSV联合感染的毒力更强。PCV-2a+PRRSV和PCV-2b+PRRSV对双感染猪的毒力均高于PCV-2e+PRRSV。与其他3种PCV-2基因型(2a、2b和2e)相比,PCV-2d的毒力增加可能是由于在PCV-2d的开放阅读框2 (ORF2)中发现了一个额外的氨基酸(赖氨酸残基)。相反,与其他3种PCV-2基因型(2a、2b和2d)相比,ORF2中额外的氨基酸可能会降低PCV-2e的毒力。本研究结果表明,PCV-2d是PRRSV共感染猪中毒性最强的PCV-2基因型。结果还表明,PCV-2基因型ORF2的遗传差异可能影响PCV-2基因型的毒力。
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来源期刊
CiteScore
1.80
自引率
0.00%
发文量
58
审稿时长
>24 weeks
期刊介绍: The Canadian Journal of Veterinary Research, published by the Canadian Veterinary Medical Association, is Canada''s only veterinary research publication. This quarterly peer-reviewed online-only journal has earned a wide international readership through the publishing of high quality scientific papers in the field of veterinary medicine. The Journal publishes the results of original research in veterinary and comparative medicine.
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