Expression of pentraxin 3 in equine lungs and neutrophils.

IF 1.1 4区 农林科学 Q2 Veterinary Canadian journal of veterinary research = Revue canadienne de recherche veterinaire Pub Date : 2023-01-01
Michelle Townsend, Brooke Fowler, Gurpreet K Aulakh, Baljit Singh
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Abstract

Endotoxin-induced diseases cause significant mortality and morbidity in the horse, leading to enormous economic damage to the equine industry. Neutrophils play a critical role in initiating the immune response in the lung. Pattern recognition receptors (PRRs) are programmed to recognize microbial structures unique to pathogens and mount an immune response. Pentraxin 3 (PTX3) is a PRR that is produced at sites of inflammation by many cell types upon stimulation by pro-inflammatory cytokines and agonists, such as endotoxins [also known as lipopolysaccharides (LPS)]. Pentraxin 3 recognizes and binds to many pathogens, activates the complement cascade, and has a role in the clearance of apoptotic and necrotic cells. Recently, PTX3 has been reported to be localized in the specific granules in human and mouse neutrophils, but no reports exist on the in-situ localization of PTX3 in neutrophils and the lungs of horses. Therefore, the objective of this study was to localize the PTX3 protein in normal and LPS-exposed neutrophils and in normal equine lungs. Immunohistochemical data showed PTX3 staining in the bronchial epithelial cells and the vascular endothelium of normal lungs. Immunogold electron microscopy localized PTX3 in the nuclei, cytoplasm, and vesicular organelles of alveolar macrophages, endothelial cells, and pulmonary intravascular macrophages. Immunohistochemical staining for PTX3 in isolated horse neutrophils showed an altered staining pattern in neutrophils stimulated with LPS. These data suggest that neutrophils may be a mobile form of PTX3 that is readily shuttled to the site of inflammation, where it can be released to fine tune a host defense response.

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戊烷素3在马肺和中性粒细胞中的表达。
内毒素引起的疾病会导致马的大量死亡率和发病率,给马业造成巨大的经济损失。中性粒细胞在启动肺部免疫反应中起关键作用。模式识别受体(PRRs)被编程为识别病原体特有的微生物结构并发起免疫反应。penttraxin 3 (PTX3)是一种PRR,在促炎细胞因子和激动剂(如内毒素[也称为脂多糖(LPS)])的刺激下,许多细胞类型在炎症部位产生。penttraxin 3识别并结合许多病原体,激活补体级联,并在凋亡和坏死细胞的清除中发挥作用。近年来,有报道称PTX3定位于人和小鼠中性粒细胞的特定颗粒中,但尚未报道PTX3在中性粒细胞和马肺中的原位定位。因此,本研究的目的是定位PTX3蛋白在正常和lps暴露的中性粒细胞和正常马肺中的位置。免疫组化结果显示正常肺支气管上皮细胞和血管内皮PTX3染色。免疫金电镜发现PTX3位于肺泡巨噬细胞、内皮细胞和肺血管内巨噬细胞的细胞核、细胞质和囊泡细胞器中。对离体马中性粒细胞PTX3的免疫组化染色显示,LPS刺激下中性粒细胞的染色模式发生改变。这些数据表明,中性粒细胞可能是PTX3的一种移动形式,它很容易穿梭到炎症部位,在那里它可以被释放以微调宿主防御反应。
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来源期刊
CiteScore
1.80
自引率
0.00%
发文量
58
审稿时长
>24 weeks
期刊介绍: The Canadian Journal of Veterinary Research, published by the Canadian Veterinary Medical Association, is Canada''s only veterinary research publication. This quarterly peer-reviewed online-only journal has earned a wide international readership through the publishing of high quality scientific papers in the field of veterinary medicine. The Journal publishes the results of original research in veterinary and comparative medicine.
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