The Effects of Clove Oil on The Biochemical and Histological Parameters, and Autophagy Markers in Polycystic Ovary Syndrome-Model Rats.

IF 2.3 Q2 OBSTETRICS & GYNECOLOGY International Journal of Fertility & Sterility Pub Date : 2023-04-01 DOI:10.22074/ijfs.2022.543640.1260
Malihe Soltani, Maryam Moghimian, Seyd-Hosein Abtahi-Evari, Seyed-Alireza Esmaeili, Ramin Mahdipour, Majid Shokoohi
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引用次数: 5

Abstract

Background: This study aimed to determine whether syzygium aromaticum (clove) could help polycystic ovary syndrome (PCOS) rats.

Materials and methods: In this experimental study, forty adult female Wistar rats (weighing 250 ± 10 g) were divided randomly into five groups; G1: control, G2: PCOS group, G3: PCOS+clove (30 mg/kg/ orally/daily) group, G4: PCOS+clove (60 mg/kg/orally/daily) group, and G5: PCOS+gonadectomy group. The PCOS was induced by a single dose injection of estradiol valerate (16 mg/kg/IM). Following PCOS induction, the rats were treated for 14 days. Histological parameters, follicle apoptosis, mRNA expression of autophagy markers (Lc3, Beclin1), oxidative stress markers, insulin and blood glucose levels, as well as serum levels of aromatase and testosterone were evaluated in these rats. Finally, the ratio of serum luteinizing hormone (LH) to follicle-stimulating hormone (FSH) levels was also calculated.

Results: The autophagy markers (Lc3, Beclin1), histological parameters, oxidative stress, insulin, and hormone levels changed significantly in the PCOS rats (G2). In G3 and G5 groups, it was observed that the levels of LH/FSH and testosterone decreased significantly in comparison to the PCOS group, and inhibition of autophagy was also observed in these groups. Treatment with cloves in the G3 group significantly improved oxidative stress, histological parameters, and insulin levels.

Conclusion: These findings demonstrated that oxidative stress, apoptosis, and excessive autophagy could be improved by treatment with low doses of clove and gonadectomy. Cloves may help to improve these parameters by regulating and inhibiting excessive autophagy. However, discovering the direct role of this extract in regulating the parameters such as oxidative stress, insulin, and androgens requires further investigation. In the present study, P<0.05 was considered statistically significant.

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丁香油对多囊卵巢综合征模型大鼠生化、组织学指标及自噬标志物的影响。
背景:本研究旨在探讨丁香对多囊卵巢综合征(PCOS)大鼠的治疗作用。材料与方法:实验选取体重250±10 g的成年雌性Wistar大鼠40只,随机分为5组;G1:对照组,G2: PCOS组,G3: PCOS+丁香(30 mg/kg/口服/日)组,G4: PCOS+丁香(60 mg/kg/口服/日)组,G5: PCOS+性腺切除术组。单剂量注射戊酸雌二醇(16 mg/kg/IM)诱导PCOS。诱导多囊卵巢综合征后,给药14 d。测定组织学参数、卵泡凋亡、自噬标志物(Lc3、Beclin1) mRNA表达、氧化应激标志物、胰岛素和血糖水平以及血清芳香化酶和睾酮水平。最后计算血清促黄体生成素(LH)与促卵泡激素(FSH)的比值。结果:PCOS大鼠自噬标志物(Lc3、Beclin1)、组织学参数、氧化应激、胰岛素、激素水平发生显著变化(G2)。在G3和G5组中,与PCOS组相比,LH/FSH和睾酮水平明显降低,自噬也受到抑制。丁香治疗G3组显著改善氧化应激、组织学参数和胰岛素水平。结论:低剂量丁香和性腺切除可改善氧化应激、细胞凋亡和过度自噬。丁香可能通过调节和抑制过度自噬来帮助改善这些参数。然而,发现这种提取物在调节氧化应激、胰岛素和雄激素等参数中的直接作用还需要进一步的研究。在本研究中,P
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来源期刊
CiteScore
4.20
自引率
0.00%
发文量
68
审稿时长
>12 weeks
期刊介绍: International Journal of Fertility & Sterility is a quarterly English publication of Royan Institute . The aim of the journal is to disseminate information through publishing the most recent scientific research studies on Fertility and Sterility and other related topics. Int J Fertil Steril has been certified by Ministry of Culture and Islamic Guidance in 2007 and was accredited as a scientific and research journal by HBI (Health and Biomedical Information) Journal Accreditation Commission in 2008. Int J Fertil Steril is an Open Access journal.
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