Potential impacts of environmental bacteria on the microbiota of loggerhead (Caretta caretta) and green (Chelonia mydas) sea turtle eggs and their hatching success

IF 3.9 3区 生物学 Q2 MICROBIOLOGY MicrobiologyOpen Pub Date : 2023-06-05 DOI:10.1002/mbo3.1363
Colleen M. McMaken, Derek A. Burkholder, Rosanna J. Milligan, Jose V. Lopez
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Abstract

Sea turtle hatching success can be affected by many variables, including pathogenic microbes, but it is unclear which microbes are most impactful and how they are transmitted into the eggs. This study characterized and compared the bacterial communities from the (i) cloaca of nesting sea turtles (ii) sand within and surrounding the nests; and (iii) hatched and unhatched eggshells from loggerhead (Caretta caretta) and green (Chelonia mydas) turtles. High throughput sequencing of bacterial 16S ribosomal RNA gene V4 region amplicons was performed on samples collected from 27 total nests in Fort Lauderdale and Hillsboro beaches in southeast Florida, United States. Significant differences were identified between hatched and unhatched egg microbiota with the differences caused predominately by Pseudomonas spp., found in higher abundances in unhatched eggs (19.29% relative abundance) than hatched eggs (1.10% relative abundance). Microbiota similarities indicate that the nest sand environment, particularly nest distance from dunes, played a larger role than the nesting mother's cloaca in influencing hatched and unhatched egg microbiota. Pathogenic bacteria potentially derive from mixed-mode transmission or additional sources not included in this study as suggested by the high proportion (24%–48%) of unhatched egg microbiota derived from unknown sources. Nonetheless, the results suggest Pseudomonas as a candidate pathogen or opportunistic colonizer associated with sea turtle egg-hatching failure.

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环境细菌对红海龟和绿海龟卵微生物群及其孵化成功率的潜在影响
海龟的孵化成功可能受到许多变量的影响,包括致病微生物,但目前尚不清楚哪些微生物影响最大,以及它们如何传播到蛋中。本研究对:(1)海龟巢腔(2)巢内和巢外沙子中的细菌群落进行了表征和比较;(iii)红海龟(Caretta Caretta)和绿海龟(Chelonia mydas)孵化和未孵化的蛋壳。对美国佛罗里达州东南部劳德代尔堡和希尔斯伯勒海滩共27个巢穴样本进行了细菌16S核糖体RNA基因V4区扩增子的高通量测序。孵蛋和未孵蛋的菌群差异显著,主要由假单胞菌引起,未孵蛋的菌群丰度(相对丰度19.29%)高于孵蛋的菌群(相对丰度1.10%)。微生物群的相似性表明,巢沙环境,特别是巢与沙丘的距离,对孵化和未孵化蛋微生物群的影响比巢母的下水道更大。病原细菌可能来自混合模式传播或本研究未包括的其他来源,未孵化的鸡蛋微生物群中有很高比例(24%-48%)来自未知来源。尽管如此,结果表明假单胞菌是与海龟蛋孵化失败相关的候选病原体或机会性殖民者。
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来源期刊
MicrobiologyOpen
MicrobiologyOpen MICROBIOLOGY-
CiteScore
8.00
自引率
0.00%
发文量
78
审稿时长
20 weeks
期刊介绍: MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era. The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes. MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to: - agriculture - antimicrobial resistance - astrobiology - biochemistry - biotechnology - cell and molecular biology - clinical microbiology - computational, systems, and synthetic microbiology - environmental science - evolutionary biology, ecology, and systematics - food science and technology - genetics and genomics - geobiology and earth science - host-microbe interactions - infectious diseases - natural products discovery - pharmaceutical and medicinal chemistry - physiology - plant pathology - veterinary microbiology We will consider submissions across unicellular and cell-cluster organisms: prokaryotes (bacteria, archaea) and eukaryotes (fungi, protists, microalgae, lichens), as well as viruses and prions infecting or interacting with microorganisms, plants and animals, including genetic, biochemical, biophysical, bioinformatic and structural analyses. The journal features Original Articles (including full Research articles, Method articles, and Short Communications), Commentaries, Reviews, and Editorials. Original papers must report well-conducted research with conclusions supported by the data presented in the article. We also support confirmatory research and aim to work with authors to meet reviewer expectations. MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.
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