Quantitative changes of brain isatin-binding proteins of rats with the rotenone-induced experimental parkinsonism.

Q3 Biochemistry, Genetics and Molecular Biology Biomeditsinskaya khimiya Pub Date : 2023-06-01 DOI:10.18097/PBMC20236903188
O A Buneeva, I G Kapitsa, L Sh Kazieva, N E Vavilov, V G Zgoda
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Abstract

Isatin (indoldione-2,3) is an endogenous regulator found in humans and animals. It exhibits a broad range of biological activity mediated by numerous isatin-binding proteins. Isatin produces neuroprotective effects in several experimental models of diseases, including Parkinsonism induced by the neurotoxin MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine).Rotenone (a neurotoxin used to modeling Parkinson's disease in rodents) causes significant changes in the profile of isatin-binding proteins of rat brain. Comparative proteomic identification of brain proteins of control rats and the rats with the rotenone-induced Parkinsonian syndrome (PS) revealed significant quantitative changes of 86 proteins under the influence of rotenone. This neurotoxin mainly caused the increase of the quantity of proteins involved in signal transduction and regulation of enzyme activity (24), proteins involved in cytoskeleton formation and exocytosis (23), and enzymes involved in energy generation and carbohydrate metabolism (19). However, only 11 of these proteins referred to isatin-binding proteins; the content of eight of them increased while the content of three proteins decreased. This suggests that the dramatic change of the profile of isatin-binding proteins, found in the development of the rotenone-induced PS, comes from changes in the state of the pre-existing molecules of proteins, rather than altered expression of corresponding genes.

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鱼藤酮诱导的实验性帕金森大鼠脑内isatin结合蛋白的定量变化。
Isatin (indoldione-2,3)是人类和动物体内发现的内源性调节剂。它表现出广泛的生物活性,由许多isatin结合蛋白介导。Isatin在几种疾病的实验模型中产生神经保护作用,包括由神经毒素MPTP(1-甲基-4-苯基-1,2,3,6-四氢吡啶)诱导的帕金森病。鱼藤酮(一种用于模拟啮齿类动物帕金森病的神经毒素)引起大鼠脑内isatin结合蛋白谱的显著变化。对照大鼠和鱼藤酮诱导的帕金森综合征(PS)大鼠的脑蛋白比较蛋白质组学鉴定显示,鱼藤酮影响下86种蛋白的数量发生了显著变化。这种神经毒素主要导致参与信号转导和酶活性调节的蛋白质(24)、参与细胞骨架形成和胞吐的蛋白质(23)以及参与能量产生和碳水化合物代谢的酶(19)的数量增加。然而,这些蛋白质中只有11种是isatin结合蛋白;其中8种蛋白质含量升高,3种蛋白质含量降低。这表明,在鱼藤酮诱导的PS的发展过程中发现的isatin结合蛋白谱的巨大变化来自于先前存在的蛋白质分子状态的变化,而不是相应基因表达的改变。
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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
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