Differential staining patterns of immunohistochemical markers for neurogenesis staging in the premature cerebellum of ferrets and mice

IF 1.3 4区 医学 Q3 PEDIATRICS Congenital Anomalies Pub Date : 2023-03-22 DOI:10.1111/cga.12513
Kazuhiko Sawada, Shiori Kamiya
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引用次数: 1

Abstract

Immunohistochemical staining patterns of markers for neurogenesis staging were compared at the identical stage of cerebellar histogenesis between ferrets (aged 20 days) and mice (aged 10 days). Proliferating cell nuclear antigen (PCNA) immunostaining was observed largely in the granular precursors of the external granular layer (EGL) in both ferrets and mice. PCNA-immunostaining was also found in brain lipid-binding protein-immunopositive cells in the internal granular layer and was more abundant in ferrets than in mice. Paired box 6 immunostaining appeared largely in the EGL granular precursors in mice, whereas it emerged in the migrating/differentiating granular precursors in ferrets. These findings revealed that the types and neurogenesis stages of the EGL granular precursors detected by immunohistochemical markers differed between ferrets and mice.

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雪貂和小鼠早期小脑神经发生分期免疫组织化学标记物的差异染色模式
比较雪貂(20日龄)和小鼠(10日龄)小脑组织发生同一阶段神经发生分期标记物的免疫组化染色模式。增殖细胞核抗原(PCNA)免疫染色主要见于雪貂和小鼠的外颗粒层(EGL)颗粒前体。在内颗粒层的脑脂结合蛋白免疫阳性细胞中也发现了pcna免疫染色,并且在雪貂中比在小鼠中更丰富。配对盒6免疫染色主要出现在小鼠的EGL颗粒前体中,而在雪貂的迁移/分化颗粒前体中出现。这些结果表明,免疫组织化学标志物检测到的EGL颗粒前体的类型和神经发生阶段在雪貂和小鼠之间存在差异。
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来源期刊
Congenital Anomalies
Congenital Anomalies PEDIATRICS-
自引率
0.00%
发文量
49
审稿时长
>12 weeks
期刊介绍: Congenital Anomalies is the official English language journal of the Japanese Teratology Society, and publishes original articles in laboratory as well as clinical research in all areas of abnormal development and related fields, from all over the world. Although contributions by members of the teratology societies affiliated with The International Federation of Teratology Societies are given priority, contributions from non-members are welcomed.
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