Magnetically Controlled 3D Cartilage Regeneration.

IF 2.7 4区医学 Q1 ORTHOPEDICS CARTILAGE Pub Date : 2024-09-01 Epub Date: 2023-07-04 DOI:10.1177/19476035231183254

Xia Chen, Ruhong Zhang, Qun Zhang, Zhicheng Xu, Feng Xu, Datao Li, Yiyuan Li

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Abstract

Objective: The cartilage regeneration field has not yet overcome the issue of effective "shaping": growing regenerated cartilage in the desired shape, and maintaining that shape, is problematic. This study reports on a new method of cartilage regeneration in which the cartilage is shaped in three dimensions. Since cartilage is composed only of cartilage cells and an abundant extracellular matrix with no blood circulation, once it is damaged, the lack of nutrient supply means that it is difficult to repair. Scaffold-free cell sheet technology plays an important role in cartilage regeneration, avoiding inflammation and immune response caused by scaffold materials. However, cartilage regenerated from the cell sheet needs to be sculpted and shaped before it can be used for cartilage defect transplantation.

Design: In this study, we used a new ultra-strong magnetic-responsive Fe3O4 nanoparticle (MNP) to shape the cartilage in vitro. Super-magnetic Fe3O4 microspheres are manufactured by co-assembling negatively charged Cetyltrimethylammonium bromide (CTAB) and positively charged Fe3+ under solvothermal conditions.

Results: The Fe3O4 MNPs are swallowed by chondrocytes, and the MNP-labeled chondrocytes are acted upon by the magnetic field. The predetermined magnetic force makes the tissues coalesce to form a multilayer cell sheet with a predetermined shape. The shaped cartilage tissue is regenerated in the transplanted body, and the nano magnetic control particles do not affect cell viability. The nanoparticles in this study improve the efficiency of cell interaction through super-magnetic modification, and to a certain extent change the way the cells absorb magnetic iron nanoparticles. This phenomenon allows a more orderly and compact alignment of the cartilage cell extracellular matrix, promotes ECM precipitation and cartilage tissue maturation, and improves the efficiency of cartilage regeneration.

Conclusion: The magnetic bionic structure, which contains specific magnetic particle-labeled cells, is deposited layer by layer to generate a three-dimensional structure with repair function, and further induce the production of cartilage. This study describes a new method for the regeneration of tissue engineered cartilage which has broad application prospects in regenerative medicine.

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磁控三维软骨再生。

目的：软骨再生领域尚未解决有效 "塑形 "的问题：将再生软骨生长成所需形状并保持该形状是个难题。本研究报告介绍了一种新的软骨再生方法，即对软骨进行三维塑形。由于软骨仅由软骨细胞和丰富的细胞外基质组成，没有血液循环，一旦受损，缺乏营养供应意味着很难修复。无支架细胞片技术在软骨再生中发挥了重要作用，避免了支架材料引起的炎症和免疫反应。然而，由细胞片再生的软骨在用于软骨缺损移植前需要雕刻和塑形：在这项研究中，我们使用了一种新型超强磁响应 Fe3O4 纳米粒子（MNP）在体外对软骨进行塑形。超强磁性的Fe3O4微球是通过在溶热条件下将带负电荷的十六烷基三甲基溴化铵（CTAB）和带正电荷的Fe3+共同组装而成的：结果：Fe3O4 MNPs 被软骨细胞吞食，MNP 标记的软骨细胞受到磁场作用。预定的磁力使组织凝聚成具有预定形状的多层细胞片。成形的软骨组织在移植体内再生，而纳米磁控颗粒不会影响细胞的活力。本研究中的纳米粒子通过超磁修饰提高了细胞相互作用的效率，并在一定程度上改变了细胞吸收磁性纳米铁粒子的方式。这种现象能使软骨细胞细胞外基质更有序、更紧密地排列，促进 ECM 沉淀和软骨组织成熟，提高软骨再生的效率：结论：磁性仿生结构含有特定的磁粉标记细胞，通过逐层沉积生成具有修复功能的三维结构，并进一步诱导软骨的生成。这项研究描述了一种组织工程软骨再生的新方法，在再生医学领域具有广阔的应用前景。

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来源期刊

CARTILAGE ORTHOPEDICS-

CiteScore

6.90

自引率

7.10%

发文量

期刊介绍： CARTILAGE publishes articles related to the musculoskeletal system with particular attention to cartilage repair, development, function, degeneration, transplantation, and rehabilitation. The journal is a forum for the exchange of ideas for the many types of researchers and clinicians involved in cartilage biology and repair. A primary objective of CARTILAGE is to foster the cross-fertilization of the findings between clinical and basic sciences throughout the various disciplines involved in cartilage repair. The journal publishes full length original manuscripts on all types of cartilage including articular, nasal, auricular, tracheal/bronchial, and intervertebral disc fibrocartilage. Manuscripts on clinical and laboratory research are welcome. Review articles, editorials, and letters are also encouraged. The ICRS envisages CARTILAGE as a forum for the exchange of knowledge among clinicians, scientists, patients, and researchers. The International Cartilage Repair Society (ICRS) is dedicated to promotion, encouragement, and distribution of fundamental and applied research of cartilage in order to permit a better knowledge of function and dysfunction of articular cartilage and its repair.