{"title":"Usefulness of measuring fast valley height rate using high-performance liquid chromatography for variant hemoglobin, Hb A2-Niigata.","authors":"Mika Ito, Masataka Shikata, Masafumi Koga","doi":"10.1007/s13340-023-00617-1","DOIUrl":null,"url":null,"abstract":"<p><p>We have reported cases of hemoglobin (Hb) A2-Niigata (δ1Val → Ala) with δ chain variant Hb and falsely high HbA1c levels. In subjects with Hb A2-Niigata, shoulder-shaped anomalous peak was detected immediately before the HbA1c peaks using high-performance liquid chromatography (HPLC; HLC-723 G9). The phenomenon where the valley between peaks of LA1c+ , a fraction containing labile HbA1c, and HbA1c fraction [fast valley (FV)] became shallow owing to the anomalous peak was observed. In this study, we attempted to quantify the height index of the FV. We assumed that the index FV height rate (FVHR) was useful for screening Hb A2-Niigata. Five subjects with Hb A2-Niigata were compared with the control group of 50 subjects without diabetes and 50 with diabetes. Various indices were measured using HPLC chromatograms, and FVHR was calculated based on these indices. FVHR in subjects with Hb A2-Niigata was significantly higher than that in subjects without and with diabetes (<i>all P</i> < <i>0.001</i>). Furthermore, when the cutoff value of FVHR was set at 23-30%, Hb A2-Niigata could be diagnosed with high sensitivity and specificity (both 100%). Hb A2-Niigata can be screened using FVHR with high sensitivity and specificity. FVHR might be also useful for screening other variant hemoglobins with abnormal HPLC chromatograph.</p>","PeriodicalId":11340,"journal":{"name":"Diabetology International","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10113408/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Diabetology International","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s13340-023-00617-1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/4/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
引用次数: 0
Abstract
We have reported cases of hemoglobin (Hb) A2-Niigata (δ1Val → Ala) with δ chain variant Hb and falsely high HbA1c levels. In subjects with Hb A2-Niigata, shoulder-shaped anomalous peak was detected immediately before the HbA1c peaks using high-performance liquid chromatography (HPLC; HLC-723 G9). The phenomenon where the valley between peaks of LA1c+ , a fraction containing labile HbA1c, and HbA1c fraction [fast valley (FV)] became shallow owing to the anomalous peak was observed. In this study, we attempted to quantify the height index of the FV. We assumed that the index FV height rate (FVHR) was useful for screening Hb A2-Niigata. Five subjects with Hb A2-Niigata were compared with the control group of 50 subjects without diabetes and 50 with diabetes. Various indices were measured using HPLC chromatograms, and FVHR was calculated based on these indices. FVHR in subjects with Hb A2-Niigata was significantly higher than that in subjects without and with diabetes (all P < 0.001). Furthermore, when the cutoff value of FVHR was set at 23-30%, Hb A2-Niigata could be diagnosed with high sensitivity and specificity (both 100%). Hb A2-Niigata can be screened using FVHR with high sensitivity and specificity. FVHR might be also useful for screening other variant hemoglobins with abnormal HPLC chromatograph.
期刊介绍:
Diabetology International, the official journal of the Japan Diabetes Society, publishes original research articles about experimental research and clinical studies in diabetes and related areas. The journal also presents editorials, reviews, commentaries, reports of expert committees, and case reports on any aspect of diabetes. Diabetology International welcomes submissions from researchers, clinicians, and health professionals throughout the world who are interested in research, treatment, and care of patients with diabetes. All manuscripts are peer-reviewed to assure that high-quality information in the field of diabetes is made available to readers. Manuscripts are reviewed with due respect for the author''s confidentiality. At the same time, reviewers also have rights to confidentiality, which are respected by the editors. The journal follows a single-blind review procedure, where the reviewers are aware of the names and affiliations of the authors, but the reviewer reports provided to authors are anonymous. Single-blind peer review is the traditional model of peer review that many reviewers are comfortable with, and it facilitates a dispassionate critique of a manuscript.