Isolation and genomic analysis of a type IV pili-independent Thermus thermophilus phage, φMN1 from a Japanese hot spring.

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of General and Applied Microbiology Pub Date : 2023-11-15 Epub Date: 2023-07-07 DOI:10.2323/jgam.2023.06.008
Masatada Tamakoshi, Atsushi Hijikata, Kei Yura, Kenshiro Oshima, Hidehiro Toh, Kaoru Mitsuoka, Tairo Oshima, Yoshitaka Bessho
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Abstract

A Thermus thermophilus lytic phage was isolated from a Japanese hot spring using a type IV pili-deficient strain as an indicator host, and designated as φMN1. Electron microscopic (EM) examination revealed that φMN1 had an icosahedral head and a contractile tail, suggesting that φMN1 belonged to Myoviridae. An EM analysis focused on φMN1 adsorption to the Thermus host cell showed that the receptor molecules for the phage were uniformly distributed on the outer surface of the cells. The circular double-stranded DNA of φMN1 was 76,659 base pairs in length, and the guanine and cytosine content was 61.8%. It was predicted to contain 99 open reading frames, and its putative distal tail fiber protein, which is essential for non-piliated host cell surface receptor recognition, was dissimilar in terms of sequence and length with its counterpart in the type IV pili-dependent φYS40. A phage proteomic tree revealed that φMN1 and φYS40 are in the same cluster, but many genes had low sequence similarities and some seemed to be derived from both mesophilic and thermophilic organisms. The gene organization suggested that φMN1 evolved from a non-Thermus phage through large-scale recombination events of the genes determining the host specificity, followed by gradual evolution by recombination of both the thermophilic and mesophilic DNAs assimilated by the host Thermus cells. This newly isolated phage will provide evolutionary insights into thermophilic phages.

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日本温泉嗜热菌φMN1的分离与基因组分析。
以一株IV型毛缺失菌株为指示宿主,从日本温泉中分离到一株嗜热热菌裂解噬菌体,命名为φMN1。电镜检查显示φMN1具有一个二十面体头部和一个可收缩的尾部,表明φMN1属于肌病毒科。对φMN1在宿主细胞上吸附的电镜分析表明,噬菌体受体分子均匀分布在细胞外表面。φMN1的环状双链DNA长度为76,659个碱基对,鸟嘌呤和胞嘧啶含量为61.8%。据预测,它含有99个开放阅读框,其推测的远端尾纤维蛋白在序列和长度方面与IV型依赖于毛的φYS40不同,而远端尾纤维蛋白是非毛状宿主细胞表面受体识别所必需的。噬菌体蛋白质组学树显示φMN1和φYS40在同一簇中,但许多基因序列相似性较低,有些基因似乎来自中温和嗜热生物。基因组织表明,φMN1是通过决定宿主特异性的基因的大规模重组事件从非热噬菌体进化而来的,随后是由宿主热细胞同化的嗜热和中温dna的重组逐渐进化而来。这种新分离的噬菌体将为嗜热噬菌体的进化提供见解。
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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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