The composition of the intestinal microbiota after allogeneic haematopoietic stem cell transplantation and its association with graft versus host disease as assessed by 16Sribosomal ribonucleic acid.

IF 2 4区 医学 Q3 PHYSIOLOGY Journal of Physiology and Pharmacology Pub Date : 2023-02-01 DOI:10.26402/jpp.2023.1.10
D-S Li, Y-R Wu, W-H Du, Y-L Zhu, W-J Zhang, Y Fu, G-B Yang
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引用次数: 1

Abstract

To observe the evolution of the intestinal microbiota in patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and discuss the relationship between the intestinal microbiota and graft-versus-host disease (GVHD). In this study, 11 patients who underwent allo-HSCT in the Aerospace Central Hospital from January 2021 to October 2021 were selected, along with 11 donors. Fecal specimens were collected 7 times: at admission, after pre-treatment, and every 3 weeks after transplantation from patients and once from donors. The composition of the intestinal microbiota and its association with GVHD after allogeneic hematopoietic stem cell transplantation were analyzed by 16S rRNA sequencing. Of the 11 patients, 5 developed GVHD, and 6 did not. The diversity of the intestinal microbiota among GVHD patients first increased and then decreased after transplantation, while that among non-GVHD patients first increased and then tended to be stable. The diversity of the intestinal microbiota among GVHD patients was lower than that among non-GVHD patients before pre-treatment and after transplantation. The taxa diversity of the intestinal microbiota in the non-GVHD group was better than that in the GVHD group before allo-HSCT, and the difference was statistically significant (P<0.05 for OTUs and CHAO1 index). The taxa abundance of Enterococcaceae 2.16% (2.13%, 2.22%) before allo-HSCT was significantly higher than that in the non-GVHD group 1.33% (0.27%, 1.52%), and the difference was statistically significant (P=0.004). There was no significant difference between the GVHD group and the non-GVHD group in the diversity of the intestinal microbiota of donors (P<0.05). The characteristics of the intestinal microbiota in the final sample of patients in the GVHD group were similar to the preoperative structure of the intestinal microbiota. In conclusion: The decrease in the diversity of the intestinal microbiota after HSCT may be a risk factor for the occurrence of GVHD. The presence of Enterococcaceae in the intestinal microbiota may be associated with an increased risk of developing GVHD. The intestinal microbiota reconstitute to be close to the intestinal microbiota composition of the donors in the non-GVHD group.

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异体造血干细胞移植后肠道微生物群的组成及其与移植物抗宿主病的关系,通过16核糖体核糖核酸进行评估。
观察同种异体造血干细胞移植(allogeneic hematopoietic stem cell transplantation, alloo - hsct)患者肠道菌群的演变,探讨肠道菌群与移植物抗宿主病(graft- anti -host disease, GVHD)的关系。在这项研究中,选择了11名于2021年1月至2021年10月在航天中心医院接受同种异体造血干细胞移植的患者,以及11名捐赠者。入院时、治疗前、移植后每3周采集患者粪便标本7次,供者粪便标本1次。采用16S rRNA测序分析同种异体造血干细胞移植后肠道菌群组成及其与GVHD的关系。在11例患者中,5例发展为GVHD, 6例没有。移植后GVHD患者肠道菌群多样性先升高后降低,非GVHD患者肠道菌群多样性先升高后趋于稳定。GVHD患者治疗前和移植后肠道菌群多样性低于非GVHD患者。同种异体造血干细胞移植前,非GVHD组肠道菌群多样性优于GVHD组,差异有统计学意义(P
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来源期刊
CiteScore
4.00
自引率
22.70%
发文量
0
审稿时长
6-12 weeks
期刊介绍: Journal of Physiology and Pharmacology publishes papers which fall within the range of basic and applied physiology, pathophysiology and pharmacology. The papers should illustrate new physiological or pharmacological mechanisms at the level of the cell membrane, single cells, tissues or organs. Clinical studies, that are of fundamental importance and have a direct bearing on the pathophysiology will also be considered. Letters related to articles published in The Journal with topics of general professional interest are welcome.
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