SARS-CoV-2 Infection Induces HMGB1 Secretion Through Post-Translational Modification and PANoptosis.

IF 4.3 4区 医学 Q2 IMMUNOLOGY Immune Network Pub Date : 2023-06-01 DOI:10.4110/in.2023.23.e26
Man Sup Kwak, Seoyeon Choi, Jiseon Kim, Hoojung Lee, In Ho Park, Jooyeon Oh, Duong Ngoc Mai, Nam-Hyuk Cho, Ki Taek Nam, Jeon-Soo Shin
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引用次数: 4

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection induces excessive pro-inflammatory cytokine release and cell death, leading to organ damage and mortality. High-mobility group box 1 (HMGB1) is one of the damage-associated molecular patterns that can be secreted by pro-inflammatory stimuli, including viral infections, and its excessive secretion levels are related to a variety of inflammatory diseases. Here, the aim of the study was to show that SARS-CoV-2 infection induced HMGB1 secretion via active and passive release. Active HMGB1 secretion was mediated by post-translational modifications, such as acetylation, phosphorylation, and oxidation in HEK293E/ACE2-C-GFP and Calu-3 cells during SARS-CoV-2 infection. Passive release of HMGB1 has been linked to various types of cell death; however, we demonstrated for the first time that PANoptosis, which integrates other cell death pathways, including pyroptosis, apoptosis, and necroptosis, is related to passive HMGB1 release during SARS-CoV-2 infection. In addition, cytoplasmic translocation and extracellular secretion or release of HMGB1 were confirmed via immunohistochemistry and immunofluorescence in the lung tissues of humans and angiotensin-converting enzyme 2-overexpressing mice infected with SARS-CoV-2.

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SARS-CoV-2感染通过翻译后修饰和PANoptosis诱导HMGB1分泌
严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)感染诱导过度的促炎细胞因子释放和细胞死亡,导致器官损伤和死亡。HMGB1 (High-mobility group box 1)是包括病毒感染在内的促炎刺激均可分泌的损伤相关分子模式之一,其分泌水平过高与多种炎性疾病有关。在这里,研究的目的是证明SARS-CoV-2感染通过主动和被动释放诱导HMGB1分泌。在SARS-CoV-2感染期间,HEK293E/ACE2-C-GFP和Calu-3细胞通过乙酰化、磷酸化和氧化等翻译后修饰介导活性HMGB1分泌。HMGB1的被动释放与多种类型的细胞死亡有关;然而,我们首次证明了PANoptosis与SARS-CoV-2感染期间HMGB1的被动释放有关,PANoptosis整合了其他细胞死亡途径,包括焦亡、凋亡和坏死亡。此外,通过免疫组织化学和免疫荧光检测,我们在感染SARS-CoV-2的人和过表达血管紧张素转换酶2的小鼠肺组织中证实了HMGB1的细胞质易位和细胞外分泌或释放。
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来源期刊
Immune Network
Immune Network Immunology and Microbiology-Immunology
CiteScore
2.90
自引率
3.30%
发文量
36
期刊介绍: Immune Network publishes novel findings in basic and clinical immunology and aims to provide a medium through which researchers in various fields of immunology can share and connect. The journal focuses on advances and insights into the regulation of the immune system and the immunological mechanisms of various diseases. Research that provides integrated insights into translational immunology is given preference for publication. All submissions are evaluated based on originality, quality, clarity, and brevity
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