Phytochemical and biological characterization of aqueous extract of Vassobia breviflora on proliferation and viability of melanoma cells: involvement of purinergic pathway.

IF 2.3 4区 医学 Q3 ENVIRONMENTAL SCIENCES Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2023-09-02 Epub Date: 2023-07-11 DOI:10.1080/15287394.2023.2233989
Altevir Rossato Viana, Nathieli Bianchin Bottari, Vinícius Rodrigues Oviedo, Daniel Santos, James Eduardo Lago Londero, Maria Rosa Chitolina Schetinger, Erico Marlon Moraes Flores, Aline Pigatto, André Passaglia Schuch, Alexandre Krause, Luciana Maria Fontanari Krause
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引用次数: 2

Abstract

Vassobia breviflora belongs to the Solanaceae family, possessing biological activity against tumor cells and is a promising alternative for therapy. The aim of this investigation was to determine the phytochemical properties V. breviflora using ESI-ToF-MS. The cytotoxic effects of this extract were examined in B16-F10 melanoma cells and the relationship if any to purinergic signaling was involved. The antioxidant activity of total phenols, (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) was analyzed, as well as production of reactive oxygen species (ROS) and nitric oxide (NO) was determined. Genotoxicity was assessed by DNA damage assay. Subsequently, the structural bioactive compounds were docked against purinoceptors P2X7 and P2Y1 receptors. The bioactive compounds found in V. breviflora were N-methyl-(2S,4 R)-trans-4-hydroxy-L-proline, calystegine B, 12-O-benzoyl- tenacigenin A and bungoside B. In vitro cytotoxicity was demonstrated at concentration ranges of 0.1-10 mg/ml, and plasmid DNA breaks only at the concentration of 10 mg/ml. V. breviflora extracts affected hydrolysis by ectoenzymes, such as ectonucleoside triphosphate diphosphohydrolase (E-NTPDase) and ectoadenosine deaminase (E-ADA) which control levels of degradation and formation of nucleosides and nucleotides. In the presence of substrates ATP, ADP, AMP and adenosine, the activities of E-NTPDase, 5´-NT or E-ADA were significantly modulated by V. breviflora. N-methyl-(2S,4 R)-trans-4-hydroxy-L-proline presented higher binding affinity (according to receptor-ligand complex estimated binding affinity as evidenced by ∆G values) to bind to both P2X7 and P2Y1purinergic receptors.Our results suggest a putative interaction of V. breviflora bioactive compounds with growth inhibitory potential in B16-F10 melanoma and suggest that may be considered as promising compounds in melanoma and cancer treatment.

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Vassobia breviflora 水提取物对黑色素瘤细胞增殖和存活能力的植物化学和生物学特性:嘌呤能途径的参与。
蒲公英(Vassobia breviflora)属于茄科植物,具有抗肿瘤细胞的生物活性,是一种很有前景的治疗方法。本研究的目的是利用 ESI-ToF-MS 方法确定面包树的植物化学特性。在 B16-F10 黑色素瘤细胞中检测了这种提取物的细胞毒性作用,以及与嘌呤能信号转导的关系。分析了总酚、2,2-二苯基-1-苦基肼(DPPH)、2,2'-偶氮-双(3-乙基苯并噻唑啉-6-磺酸(ABTS))的抗氧化活性,以及活性氧(ROS)和一氧化氮(NO)的产生情况。遗传毒性通过 DNA 损伤试验进行评估。随后,对结构性生物活性化合物与嘌呤受体 P2X7 和 P2Y1 受体进行了对接。在蒲公英中发现的生物活性化合物有 N-甲基-(2S,4R)-反式-4-羟基-L-脯氨酸、钙苷元 B、12-O-苯甲酰基-tenacigenin A 和 bungoside B,体外细胞毒性的浓度范围为 0.1-10 mg/ml,质粒 DNA 断裂的浓度仅为 10 mg/ml。蒲公英提取物会影响外切酶的水解作用,如控制核苷和核苷酸降解和形成水平的外切核苷三磷酸二磷酸水解酶(E-NTPDase)和外切腺苷脱氨酶(E-ADA)。在底物 ATP、ADP、AMP 和腺苷存在的情况下,E-NTPDase、5´-NT 或 E-ADA 的活性受蒲公英的影响很大。我们的研究结果表明,V. breviflora 的生物活性化合物与 B16-F10 黑色素瘤的生长抑制潜能之间存在潜在的相互作用,并建议将其作为治疗黑色素瘤和癌症的有前途的化合物。
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来源期刊
CiteScore
5.20
自引率
19.20%
发文量
46
审稿时长
8-16 weeks
期刊介绍: The Journal of Toxicology and Environmental Health, Part A , Current Issues is an authoritative journal that features strictly refereed original research in the field of environmental sciences, public and occupational health, and toxicology.
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