Disease-related biomarkers as experimental endpoints in 3D skin culture models.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2023-06-01 Epub Date: 2023-04-03 DOI:10.1007/s10616-023-00574-2

Deepa Chaturvedi, Swarali Paranjape, Ratnesh Jain, Prajakta Dandekar

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Abstract

The success of in vitro 3D models in either recapitulating the normal tissue physiology or altered physiology or disease condition depends upon the identification and/or quantification of relevant biomarkers that confirm the functionality of these models. Various skin disorders, such as psoriasis, photoaging, vitiligo, etc., and cancers like squamous cell carcinoma and melanoma, etc. have been replicated via organotypic models. The disease biomarkers expressed by such cell cultures are quantified and compared with the biomarkers expressed in cultures depicting the normal tissue physiology, to identify the most prominent variations in their expression. This may also indicate the stage or reversal of these conditions upon treatment with relevant therapeutics. This review article presents an overview of the important biomarkers that have been identified in in-vitro 3D models of skin diseases as endpoints for validating the functionality of these models.

Supplementary information: The online version contains supplementary material available at 10.1007/s10616-023-00574-2.

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疾病相关生物标志物作为3D皮肤培养模型中的实验终点。

体外3D模型在概括正常组织生理学或改变的生理学或疾病状况方面的成功取决于确认这些模型功能的相关生物标志物的识别和/或量化。各种皮肤疾病，如银屑病、光老化、白癜风等，以及鳞状细胞癌和黑色素瘤等癌症，已经通过器官型模型复制。对这种细胞培养物表达的疾病生物标志物进行量化，并将其与描述正常组织生理学的培养物中表达的生物标志物相比较，以确定其表达中最显著的变化。这也可能表明在用相关疗法治疗后这些状况的阶段或逆转。这篇综述文章概述了在体外皮肤病3D模型中鉴定的重要生物标志物，这些生物标志物是验证这些模型功能的终点。补充信息：在线版本包含补充材料，可访问10.1007/s10616-023-00574-2。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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