Use of Mesenchymal Stem Cells in Experimental Ovarian Damage.

IF 2.1 4区医学 Q4 CELL & TISSUE ENGINEERING Current stem cell research & therapy Pub Date : 2024-01-01 DOI:10.2174/1574888X18666230713121530

Hanan Fouad, Ibrahim A Albahlol, Hazim A Wahab, Eman H Nadwa, Heba M Galal, Mohamed Abouelkheir, Ahmed E Taha, Abdelkarim G Kamel, Hassan A Abdelmawlla

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Abstract

Background: Bisphenol-A (BPA) has a well-proven deleterious effect on the hypothalamicpituitary- gonadal axis.

Objectives: The current study investigated the therapeutic potentials of mesenchymal stem cells (MSCs) in a murine model of BPA-induced ovarian damage.

Methods: Fifty adult female rats were divided into: Group 1; control group, Group IIa, IIb: rats were given oral gavage of BPA (25 and 50 mg/Kg body weight respectively) on a daily basis for 15 days, and Group IIIa, IIIb; rats were intravenously treated with of MSCs (10⁶ cells) after receiving the last dose of BPA as in group II. Plasma and ovarian tissue levels of Malondialdehyde (MDA) and gonadal axis hormones were assessed. Apoptosis was evaluated by TUNNEL assay and by apoptosis markers (FAS, FASL, Caspase 3, SLTM). A histological examination of ovarian tissue was also conducted.

Results: BPA resulted in a significant elevation in plasma levels of LH, FSH, and ovarian tissue levels of MDA and a significant decrease in estradiol and progesterone. All genetic and protein markers of apoptosis were elevated in BPA treated group with decreased oestrogen receptor expression in the ovarian tissue. Increased apoptotic cells were confirmed by TUNEL assay. A high dose of BPA was able to increase the number of atretic follicles in the ovarian tissue whereas the numbers of primordial, primary, secondary and Graafian follicles were decreased. All the laboratory and histological abnormalities were ameliorated by treatment with MSCs.

Conclusion: The antioxidant and anti-apoptotic effects of MSCs could possibly explain the ability of this therapeutic modality to ameliorate BPA-induced-ovarian damage.

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在实验性卵巢损伤中使用间充质干细胞。

背景：双酚A（BPA）对下丘脑-垂体-性腺轴的有害影响已被证实：本研究探讨了间充质干细胞（MSCs）在双酚 A 诱导的卵巢损伤小鼠模型中的治疗潜力：方法：将50只成年雌性大鼠分为两组：方法：将50只成年雌性大鼠分为：对照组（第1组）；第IIa、IIb组：每天给大鼠灌胃双酚A（分别为25和50毫克/千克体重），持续15天；第IIIa、IIIb组：在接受最后一次双酚A剂量后，给大鼠静脉注射间充质干细胞（106个细胞），方法同第II组。评估血浆和卵巢组织中丙二醛（MDA）和性腺轴激素的水平。通过 TUNNEL 试验和细胞凋亡标志物（FAS、FASL、Caspase 3、SLTM）对细胞凋亡进行评估。还对卵巢组织进行了组织学检查：结果：双酚 A 会导致血浆中 LH、FSH 水平和卵巢组织中 MDA 水平明显升高，而雌二醇和孕酮则明显下降。在双酚 A 处理组中，所有细胞凋亡的基因和蛋白质标记物均升高，卵巢组织中的雌激素受体表达减少。TUNEL 试验证实了凋亡细胞的增加。高剂量的双酚 A 能增加卵巢组织中闭锁卵泡的数量，而原始卵泡、初级卵泡、次级卵泡和 Graafian 卵泡的数量则有所减少。用间叶干细胞治疗后，所有实验室和组织学异常都得到了改善：结论：间充质干细胞的抗氧化和抗凋亡作用可能是这种治疗方法能够改善双酚 A 诱导的卵巢损伤的原因。

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来源期刊

Current stem cell research & therapy CELL & TISSUE ENGINEERING-CELL BIOLOGY

CiteScore

4.20

自引率

3.70%

发文量

197

审稿时长

>12 weeks

期刊介绍： Current Stem Cell Research & Therapy publishes high quality frontier reviews, drug clinical trial studies and guest edited issues on all aspects of basic research on stem cells and their uses in clinical therapy. The journal is essential reading for all researchers and clinicians involved in stem cells research.