Natalia A. Carrion , Rodolfo E. Quiroz , Camila Asenzo , Manuela Gamarra , Antonio E. Flores , Maria Garcia , Rolando N. Soloaga , Julia Grigioni , Maria Mastroianni , Alejandra Margari , Facundo Molina , Adriana N. Procopio , Vanesa Reijtman , Maria S. Ratti , Victor Mamani , Myriam Vazquez , Diana Viale , Guadalupe Perez
{"title":"Optimización en la detección de bacteriemias. Uso integrado de FilmArray (BCID2) y MALDI-TOF","authors":"Natalia A. Carrion , Rodolfo E. Quiroz , Camila Asenzo , Manuela Gamarra , Antonio E. Flores , Maria Garcia , Rolando N. Soloaga , Julia Grigioni , Maria Mastroianni , Alejandra Margari , Facundo Molina , Adriana N. Procopio , Vanesa Reijtman , Maria S. Ratti , Victor Mamani , Myriam Vazquez , Diana Viale , Guadalupe Perez","doi":"10.1016/j.ram.2023.06.001","DOIUrl":null,"url":null,"abstract":"<div><p>The usefulness of the combined use of MALDI-TOF MS from a subculture with 3-5<!--> <!-->h of incubation and the BCID2 panel (FilmArray) for the identification of microorganisms from positive blood cultures and its importance in the adjustment of antimicrobial therapy was analyzed. Overall identification with BCID2 was 90.4% (142/157) and with Maldi-TOF MS 83.4% (131/157) (<em>p</em> <!-->=<!--> <!-->0.0858); in 23 polymicrobial episodes (47 strains), the BCID2 panel identified 45 (95.7%) and MALDI-TOF MS 24 (51.1%) (<em>p<!--> </em><<!--> <!-->0.0000). BCID2 detected the presence of the resistance genes <em>mec</em>A/C (n<!--> <!-->=<!--> <!-->16), <em>bla</em><sub>KPC</sub> (n<!--> <!-->=<!--> <!-->8); <em>bla</em><sub>CTX-M</sub> (n<!--> <!-->=<!--> <!-->17), <em>bla</em><sub>NDM</sub> (n<!--> <!-->=<!--> <!-->8), <em>bla</em><sub>OXA-48</sub> (n<!--> <!-->=<!--> <!-->1), and <em>van</em>A/B (n<!--> <!-->=<!--> <!-->2). The median time to report a result was 2.0<!--> <!-->h for BCID2 and 4.0<!--> <!-->h for MALDI-TOF MS (<em>p</em> <!--><<!--> <!-->0.0000). Of 124 episodes analyzed, the rapid result of BCID2 led to 82.3% (102/124) therapeutic changes.</p></div>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000524/pdfft?md5=9d1376e994dfb7eaf4494b58887faf4f&pid=1-s2.0-S0325754123000524-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0325754123000524","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
The usefulness of the combined use of MALDI-TOF MS from a subculture with 3-5 h of incubation and the BCID2 panel (FilmArray) for the identification of microorganisms from positive blood cultures and its importance in the adjustment of antimicrobial therapy was analyzed. Overall identification with BCID2 was 90.4% (142/157) and with Maldi-TOF MS 83.4% (131/157) (p = 0.0858); in 23 polymicrobial episodes (47 strains), the BCID2 panel identified 45 (95.7%) and MALDI-TOF MS 24 (51.1%) (p < 0.0000). BCID2 detected the presence of the resistance genes mecA/C (n = 16), blaKPC (n = 8); blaCTX-M (n = 17), blaNDM (n = 8), blaOXA-48 (n = 1), and vanA/B (n = 2). The median time to report a result was 2.0 h for BCID2 and 4.0 h for MALDI-TOF MS (p < 0.0000). Of 124 episodes analyzed, the rapid result of BCID2 led to 82.3% (102/124) therapeutic changes.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
