Polymorphonuclear phenotypical expression of CD18, at baseline and after in vitro activation, in several clinical disorders: Revision of our case series.

IF 2.1 4区医学 Q3 HEMATOLOGY Clinical hemorheology and microcirculation Pub Date : 2023-01-01 DOI:10.3233/CH-231771

Gregorio Caimi, Rosalia Lo Presti, Caterina Carollo, Maria Montana, Melania Carlisi

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Abstract

Background: In relation to the different and important roles of the beta2 integrins, we have revisited the expression of polymorphonuclear leukocyte CD18 in several clinical disorders, at baseline and after in vitro activation.

Subjects: we have examined subjects with type 1 diabetes mellitus, vascular atherosclerotic disease, type 2 diabetes mellitus without and with macrovascular complications, chronic renal failure on conservative treatment, essential hypertension, deep venous thrombosis, acute ischemic stroke and subjects with venous leg ulcers.

Methods: unfractioned leukocyte suspension was prepared according to the Mikita's method, while the leukocyte were separated into mononuclear and polymorphonuclear cells with a Ficoll-Hypaque medium. Using specific monoclonal antibody, the CD18 expression was evaluated with cytofluorimetric analysis, using FACScan (Becton Dickinson) be Cellquest software; the activation in vitro with PMA was effected according to modified Yasui and Masuda methods.

Results: in type 1 diabetes mellitus, at baseline CD18 is under expressed in comparison with normal control, and not changes after PMA activation were observed; in subjects with vascular atherosclerotic disease, in type 2 diabetes mellitus CD18 is over expressed at baseline but does not vary after activation; in subjects with chronic renal failure, essential hypertension and in subjects with acute ischemic stroke the CD18 up-regulate at baseline compared to normal control, and it increases further after activation; in subjects with deep venous thrombosis the CD18 expression is not different from control group at baseline, but it increases after activation; finally, in subjects with venous leg ulcers the CD18 is normally expressed at baseline, and it does not change after PMA activation.

Conclusions: in the different clinical disorders, the trend of this integrin subunit provides some specific information, useful to select the best therapeutic strategy in clinical practice.

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CD18在基线和体外激活后在几种临床疾病中的多形核表型表达：我们病例系列的修订。

背景：关于β2整合素的不同和重要作用，我们重新研究了多形核白细胞CD18在几种临床疾病中的表达，在基线和体外激活后。受试者：我们检查了患有1型糖尿病、血管动脉粥样硬化性疾病、无大血管并发症和伴有大血管并发症的2型糖尿病、保守治疗的慢性肾功能衰竭、原发性高血压、深静脉血栓形成、急性缺血性脑卒中和下肢静脉溃疡的受试者。方法：按照Mikita的方法制备普通白细胞悬浮液，用Ficoll-Hypaque培养基将白细胞分离为单核细胞和多形核细胞。使用特异性单克隆抗体，使用FACScan（Becton Dickinson）be Cellquest软件通过细胞荧光分析评估CD18的表达；根据改良的Yasui和Masuda方法，用PMA进行体外活化。结果：在1型糖尿病中，与正常对照组相比，CD18在基线时表达不足，并且在激活PMA后没有观察到变化；在患有血管动脉粥样硬化疾病的受试者中，在2型糖尿病中，CD18在基线时过度表达，但在激活后没有变化；在患有慢性肾功能衰竭、原发性高血压和急性缺血性中风的受试者中，与正常对照组相比，CD18在基线时上调，并且在激活后进一步增加；在深静脉血栓形成的受试者中，CD18的表达在基线时与对照组没有差异，但在激活后增加；最后，在患有下肢静脉性溃疡的受试者中，CD18在基线时正常表达，并且在PMA激活后没有改变。结论：在不同的临床疾病中，整合素亚基的变化趋势提供了一些特定的信息，有助于在临床实践中选择最佳的治疗策略。

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来源期刊

Clinical hemorheology and microcirculation 医学-外周血管病

CiteScore

4.30

自引率

33.30%

发文量

170

期刊介绍： Clinical Hemorheology and Microcirculation, a peer-reviewed international scientific journal, serves as an aid to understanding the flow properties of blood and the relationship to normal and abnormal physiology. The rapidly expanding science of hemorheology concerns blood, its components and the blood vessels with which blood interacts. It includes perihemorheology, i.e., the rheology of fluid and structures in the perivascular and interstitial spaces as well as the lymphatic system. The clinical aspects include pathogenesis, symptomatology and diagnostic methods, and the fields of prophylaxis and therapy in all branches of medicine and surgery, pharmacology and drug research. The endeavour of the Editors-in-Chief and publishers of Clinical Hemorheology and Microcirculation is to bring together contributions from those working in various fields related to blood flow all over the world. The editors of Clinical Hemorheology and Microcirculation are from those countries in Europe, Asia, Australia and America where appreciable work in clinical hemorheology and microcirculation is being carried out. Each editor takes responsibility to decide on the acceptance of a manuscript. He is required to have the manuscript appraised by two referees and may be one of them himself. The executive editorial office, to which the manuscripts have been submitted, is responsible for rapid handling of the reviewing process. Clinical Hemorheology and Microcirculation accepts original papers, brief communications, mini-reports and letters to the Editors-in-Chief. Review articles, providing general views and new insights into related subjects, are regularly invited by the Editors-in-Chief. Proceedings of international and national conferences on clinical hemorheology (in original form or as abstracts) complete the range of editorial features.