{"title":"The Battle Against Quenching and Fading for Fluorescence: A Model of How to Evaluate the Fluorophore's Behavior.","authors":"Gloria E Hoffman","doi":"10.1369/00221554231185184","DOIUrl":null,"url":null,"abstract":"<p><p>The fading and quenching of fluorescence intensity has been a major problem in the use of fluorescein isothiocyanate (FITC) for immunofluorescence cytochemical techniques, especially with laser confocal microscopy. The companion article by Longin et al. provided an empirical approach to overcoming this problem. The present commentary highlights the significance of the Longin et al. article when it was published and its continued relevance today.</p>","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":"71 7","pages":"409-410"},"PeriodicalIF":1.9000,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10363908/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Histochemistry & Cytochemistry","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1369/00221554231185184","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/7/7 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The fading and quenching of fluorescence intensity has been a major problem in the use of fluorescein isothiocyanate (FITC) for immunofluorescence cytochemical techniques, especially with laser confocal microscopy. The companion article by Longin et al. provided an empirical approach to overcoming this problem. The present commentary highlights the significance of the Longin et al. article when it was published and its continued relevance today.
期刊介绍：
Journal of Histochemistry & Cytochemistry (JHC) has been a pre-eminent cell biology journal for over 50 years. Published monthly, JHC offers primary research articles, timely reviews, editorials, and perspectives on the structure and function of cells, tissues, and organs, as well as mechanisms of development, differentiation, and disease. JHC also publishes new developments in microscopy and imaging, especially where imaging techniques complement current genetic, molecular and biochemical investigations of cell and tissue function. JHC offers generous space for articles and recognizing the value of images that reveal molecular, cellular and tissue organization, offers free color to all authors.
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