Transcription factor ETV1-induced lncRNA MAFG-AS1 promotes migration, invasion, and epithelial-mesenchymal transition of pancreatic cancer cells by recruiting IGF2BP2 to stabilize ETV1 expression.

IF 1.8 4区生物学 Q4 CELL BIOLOGY Growth factors Pub Date : 2023-08-01 Epub Date: 2023-07-10 DOI:10.1080/08977194.2023.2227272

Hanqin Weng, Weijian Feng, Fengling Li, Dong Huang, Liangyi Lin, Zaiguo Wang

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Abstract

We investigated the mechanism of ETS-translocation variant 1 (ETV1)/lncRNA-MAFG-AS1 in pancreatic cancer (PC). MAFG-AS1 and ETV1 levels in PC cell lines and HPNE cells were determined using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blotting (WB). After transfection with sh-MAFG-AS1, PC cell invasion, migration, proliferation, and epithelial-mesenchymal transition (EMT)-related proteins were measured by 5-ethynyl-2'-deoxyuridine (EdU), Transwell assay, and WB. The binding between ETV1 and MAFG-AS1 was studied using dual-luciferase assay and chromatin immunoprecipitation. The interactions between MAFG-AS1, IGF2BP2, and ETV1 were tested. Combined experiments were further performed using sh-MAFG-AS1 and pcDNA-ETV1 simultaneously. ETV1/MAFG-AS1 was highly expressed in PC cells. Blocking MAFG-AS1 inhibited the malignant behaviors of PC cells. ETV1 induced MAFG-AS1 transcription in PC cells. MAFG-AS1 stabilized ETV1 mRNA by recruiting IGF2BP2. ETV1 overexpression partially antagonized the suppression of silencing MAFG-AS1 on PC cells. ETV1-induced MAFG-AS1 stabilized the ETV1 expression by recruiting IGF2BP2 and promoted PC cell migration, invasion, proliferation, and EMT.

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转录因子ETV1诱导的lncRNA MAFG-AS1通过招募IGF2BP2来稳定ETV1的表达，从而促进胰腺癌细胞的迁移、侵袭和上皮-间质转化。

我们研究了ETS-转移变体1（ETV1）/lncRNA-MAFG-AS1在胰腺癌（PC）中的作用机制。我们采用反转录定量聚合酶链反应（RT-qPCR）和免疫印迹（WB）技术测定了PC细胞系和HPNE细胞中的MAFG-AS1和ETV1水平。转染 sh-MAFG-AS1 后，通过 5- 乙炔基-2'-脱氧尿苷（EdU）、Transwell 试验和 WB 检测了 PC 细胞的侵袭、迁移、增殖和上皮-间质转化（EMT）相关蛋白。使用双荧光素酶检测法和染色质免疫沉淀法研究了ETV1和MAFG-AS1之间的结合。检测了 MAFG-AS1、IGF2BP2 和 ETV1 之间的相互作用。同时使用 sh-MAFG-AS1 和 pcDNA-ETV1 进一步进行了联合实验。ETV1/MAFG-AS1在PC细胞中高表达。阻断MAFG-AS1可抑制PC细胞的恶性行为。ETV1诱导PC细胞中MAFG-AS1的转录。MAFG-AS1通过招募IGF2BP2稳定ETV1 mRNA。ETV1的过表达部分拮抗了沉默MAFG-AS1对PC细胞的抑制作用。ETV1诱导的MAFG-AS1通过招募IGF2BP2稳定了ETV1的表达，并促进了PC细胞的迁移、侵袭、增殖和EMT。

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来源期刊

Growth factors 生物-内分泌学与代谢

CiteScore

2.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Growth Factors is an international and interdisciplinary vehicle publishing new knowledge and findings on the regulators of cell proliferation, differentiation and survival. The Journal will publish research papers, short communications and reviews on current developments in cell biology, biochemistry, physiology or pharmacology of growth factors, cytokines or hormones which improve our understanding of biology or medicine. Among the various fields of study topics of particular interest include: •Stem cell biology •Growth factor physiology •Structure-activity relationships •Drug development studies •Clinical applications