FSCN1 has a potential indication for the prognosis and regulates the migration of HNSCC.

IF 2.2 4区医学 Q3 ONCOLOGY Cancer Biomarkers Pub Date : 2023-01-01 DOI:10.3233/CBM-220409

Yuliang Zhang, Anyan Zhou, Jiabin Nian, Shuzhou Liu, Xin Wei

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Abstract

Background: The study of molecular markers for diagnosis and prognosis is of great clinical significance for HNSCC patients. In this study, we proposed that FSCN1 has a potential indication for prognosis and is essential for the migration of HNSCC.

Methods: We analyzed the expression and survival association of FSCN1 in HNSCC using TCGA data. We compared the expression of FSCN1 in tumors from primary and metastasis HNSCC patients using QPCR, western blotting, and immunochemistry staining. We determined the migration velocity of multiple HNSCC cell lines using a chemotaxis migration assay. We analyzed the correlation between FSCN1 expression and HNSCC cell migration. We also test the effect of FSCN1 knockdown and overexpression on HNSCC cell migration.

Results: FSCN1 was overexpressed in HNSCC than pair normal tissues and metastasis HNSCC than primary HNSCC. FSCN1 expression was associated with significantly poorer overall survival of HNSCC patients. FSCN1 was potentially associated with immune cell infiltration and migration-associated genes. FSCN1 level was correlated with the migration in HNSCC cell lines. Knockdown of FSCN1 reduced the migration and the overexpression of FSCN1 promoted the migration of HNSCC cell lines.

Conclusion: FSCN1 is a potential prognostic marker and a critical biomolecule for the migration of HNSCC.

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FSCN1对HNSCC的预后具有潜在的指示作用，并调节其迁移。

背景：研究分子标记物对HNSCC患者的诊断和预后具有重要的临床意义。在本研究中，我们提出FSCN1具有潜在的预后指征，并且对HNSCC的迁移至关重要。方法：我们使用TCGA数据分析了FSCN1在HNSCC中的表达和生存相关性。我们使用QPCR、蛋白质印迹和免疫化学染色比较了FSCN1在原发性和转移性HNSCC患者肿瘤中的表达。我们使用趋化性迁移测定法测定了多个HNSCC细胞系的迁移速度。我们分析了FSCN1表达与HNSCC细胞迁移之间的相关性。我们还测试了FSCN1敲低和过表达对HNSCC细胞迁移的影响。结果：FSCN1在HNSCC中的表达高于配对正常组织，转移性HNSCC的表达高于原发性HNSCC。FSCN1的表达与HNSCC患者的总体生存率显著降低相关。FSCN1可能与免疫细胞浸润和迁移相关基因相关。FSCN1水平与HNSCC细胞系的迁移有关。敲低FSCN1减少了HNSCC细胞系的迁移，而过表达FSCN1促进了HNSCC的迁移。结论：FSCN1是一种潜在的预后标志物，也是HNSCC迁移的关键生物分子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer Biomarkers ONCOLOGY-

CiteScore

5.20

自引率

3.20%

发文量

195

审稿时长

3 months

期刊介绍： Concentrating on molecular biomarkers in cancer research, Cancer Biomarkers publishes original research findings (and reviews solicited by the editor) on the subject of the identification of markers associated with the disease processes whether or not they are an integral part of the pathological lesion. The disease markers may include, but are not limited to, genomic, epigenomic, proteomics, cellular and morphologic, and genetic factors predisposing to the disease or indicating the occurrence of the disease. Manuscripts on these factors or biomarkers, either in altered forms, abnormal concentrations or with abnormal tissue distribution leading to disease causation will be accepted.