Discovery of the First Lytic Staphylococcus pseudintermedius/Staphylococcus aureus Polyvalent Bacteriophages.

PHAGE (New Rochelle, N.Y.) Pub Date : 2022-06-01 Epub Date: 2022-06-16 DOI:10.1089/phage.2022.0004

Haroldo Hernandez Santos, Justin Clark, Austen Terwilliger, Anthony Maresso

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Abstract

Background: There are no verified lytic Staphylococcus pseudintermedius phages in the literature and few temperate phage genomes in databases. S. pseudintermedius is an opportunistic zoonotic pathogen of great importance in veterinary and human medicine.

Materials and methods: We discovered phages against canine-derived S. pseudintermedius isolates by screening dog feces, hair, and skin swabs. Fourteen new phages were isolated and characterized by genomic analysis, transmission electron microscopy, and host range determination.

Results: Three phages-DH2, DH5, and DS10, a phage K variant-were predicted lytic by sequencing, a designation supported by mitomycin C induction. All three are S. pseudintermedius and Staphylococcus aureus polyvalent phages, with DH2 and DS10 being strong killers of both species.

Conclusions: We report discovery of the first verified lytic S. pseudintermedius phages and suggest dog hair as a novel reservoir. DH2, DH5, and DS10 are promising candidates toward developing an anti-Staphylococcal phage cocktail.

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发现首个溶解性假中间葡萄球菌/金黄色葡萄球菌多价噬菌体。

背景：文献中没有经过验证的溶解性假中间葡萄球菌噬菌体，数据库中的温带噬菌体基因组也很少。伪中间葡萄球菌是一种机会性人畜共患病原体，在兽医和人类医学中具有重要意义：我们通过筛查狗的粪便、毛发和皮肤拭子，发现了针对源于犬的伪间皮细胞分离物的噬菌体。通过基因组分析、透射电子显微镜和宿主范围测定，我们分离并鉴定了 14 种新噬菌体：结果：三个噬菌体--DH2、DH5和DS10（噬菌体K的变种）--通过测序被预测为具有溶解性，丝裂霉素C诱导证实了这一说法。这三种噬菌体都是伪中间葡萄球菌和金黄色葡萄球菌的多价噬菌体，其中 DH2 和 DS10 对这两种葡萄球菌都有很强的杀伤力：结论：我们报告发现了首个经过验证的致死性假中间葡萄球菌噬菌体，并认为狗毛是一个新的贮藏库。DH2、DH5 和 DS10 是开发抗葡萄球菌噬菌体鸡尾酒的有希望的候选噬菌体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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PHAGE (New Rochelle, N.Y.)

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