Veterinary Management of Harderian Gland Tumors in Cancer Rainbow (crainbow) HER2-Positive Mice.

IF 1.3 4区农林科学 Q2 VETERINARY SCIENCES Comparative medicine Pub Date : 2022-12-01 DOI:10.30802/AALAS-CM-22-000061

Angela Garner, Joshua D Ginzel, Joshua C Snyder, Jeffrey I Everitt, Chelsea D Landon

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Abstract

A Cancer Rainbow mouse line that expresses 3 fluorescently labeled isoforms of the tumor-driver gene HER2 (HER2BOW) was developed recently for the study of tumorigenesis in the mammary gland. The expression of 1 of the 3 HER2 isoforms in HER2BOW mice is induced through the Cre/lox system. However, in addition to developing palpable mammary tumors, HER2BOW mice developed orbital tumors, specifically of the Harderian gland. Mice were euthanized, and histopathologic examination of the Harderian gland tumors was performed. Tumors were characterized by adenomatous hyperplasia to multinodular adenomas of the Harderian gland. Fluorescent imaging of the Harderian gland tissue confirmed the expression of HER2 in the tumors. Here we discuss monitoring and palliative approaches to allow attainment of humane experimental endpoints of mammary tumor growth in this mouse line. We describe a range of interventions, including close monitoring, topical palliative care, and surgical bilateral enucleation. Based on our data and previous reports in the literature, the overexpression of HER2 in Harderian gland tissue and subsequent tumor formation likely was driven by MMTV-Cre expression in the Harderian gland.

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癌症 Rainbow (crainbow) HER2 阳性小鼠硬腺肿瘤的兽医管理。

为研究乳腺肿瘤发生，最近开发出了一种表达肿瘤驱动基因 HER2 的 3 种荧光标记异构体的 Cancer Rainbow 小鼠品系（HER2BOW）。HER2BOW 小鼠中 3 种 HER2 异构体中 1 种的表达是通过 Cre/lox 系统诱导的。然而，HER2BOW 小鼠除了出现可触及的乳腺肿瘤外，还出现了眼眶肿瘤，特别是哈德氏腺肿瘤。小鼠被安乐死后，对哈德氏腺肿瘤进行了组织病理学检查。肿瘤的特征是哈氏腺腺瘤性增生到多结节腺瘤。哈氏腺组织的荧光成像证实了肿瘤中HER2的表达。在此，我们讨论了监测和缓解方法，以便在该小鼠品系中实现乳腺肿瘤生长的人道实验终点。我们介绍了一系列干预措施，包括密切监测、局部姑息治疗和外科双侧去核手术。根据我们的数据和以前的文献报道，哈氏腺组织中 HER2 的过度表达以及随后肿瘤的形成可能是由哈氏腺中 MMTV-Cre 的表达驱动的。

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来源期刊

Comparative medicine 医学-动物学

CiteScore

1.90

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.