Could vitamin D protect against high fat diet induced damage in the arcuate nucleus in the rat: histological, immunohistochemical and ultrastructural study.

IF 1.1 4区 医学 Q4 MICROSCOPY Ultrastructural Pathology Pub Date : 2023-07-04 DOI:10.1080/01913123.2023.2195484
Hala Mohamed Hassanin, Omnia I Ismail
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Abstract

Obesity is a serious health issue. As regard, the central nervous system, obesity induces neuronal damage. Vitamin D has well-known anti-inflammatory and neuroprotective effects. To detect if vitamin D protects against damage in the arcuate nucleus induced by a high fat-high fructose diet. Forty adult rats were used, and four groups were formed. Group I (negative control) kept on a standard chow diet for six weeks, Group II (positive control) received vitamin D orally once every other day for six weeks, Group III (high fat-high fructose treated group) was given high fat-high fructose diets for six weeks and Group IV (high fat-high fructose and vitamin D treated group) were given high fat-high fructose diets concomitantly with vitamin D for six weeks. High fat-high fructose diet markedly caused histological changes in arcuate neurons as nuclei appeared darkly stained and shrunken with condensed chromatin, and the nucleolus became less prominent. The cytoplasm appeared rarefied with loss of most of the organelles. An increase in neuroglial cells was noticed. The synaptic area showed sparse degenerated mitochondria and a disrupted presynaptic membrane. A high-fat diet has a damaging effect on arcuate neurons and vitamin D alleviates these effects.

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维生素D对高脂饮食对大鼠弓状核损伤的保护作用:组织学、免疫组化和超微结构研究。
肥胖是一个严重的健康问题。就中枢神经系统而言,肥胖会引起神经元损伤。维生素D具有众所周知的抗炎和神经保护作用。检测维生素D是否能防止高脂高果糖饮食引起的弓形核损伤。选用40只成年大鼠,分为四组。ⅰ组(阴性对照)给予标准鼠粮6周,ⅱ组(阳性对照)每隔一天口服一次维生素D,连续6周,ⅲ组(高脂肪-高果糖处理组)给予高脂肪-高果糖饮食6周,ⅳ组(高脂肪-高果糖和维生素D处理组)给予高脂肪-高果糖饮食同时给予维生素D 6周。高脂高果糖饮食明显引起弓形神经元的组织学改变,细胞核出现黑色染色和萎缩,染色质浓缩,核仁变得不那么突出。细胞质稀薄,大部分细胞器丢失。观察到神经胶质细胞增多。突触区线粒体稀疏退化,突触前膜断裂。高脂肪饮食对弓状神经元有破坏性影响,维生素D可以减轻这种影响。
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来源期刊
Ultrastructural Pathology
Ultrastructural Pathology 医学-病理学
CiteScore
2.00
自引率
10.00%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.
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