The Effect of The Conditioned Medium from Human Embryonic Stem Cells on Mouse Oocytes In Vitro Maturation.

IF 1.7 4区生物学 Q4 CELL BIOLOGY Cell Journal Pub Date : 2023-07-25 DOI:10.22074/cellj.2023.1987024.1217

Roshan Rezaee Ranjbar Sardari, Mahmood Dehghani Ashkezari, Seyed Morteza Seifati, Mahin Izadi, Jalal Golzadeh, Fahime Mazaheri, Maryam Adib, Behrouz Aflatoonian

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Abstract

Objective: Some reports have indicated that conditioned medium from growing mouse embryonic stem cells (ESCs) provides a supportive condition for small follicles growing, oocyte maturation, and following embryo growth. The aim of this study is assessing in vitro maturation (IVM) and consequent in vitro fertilization (IVF) outcome of immature mouse oocytes using human embryonic stem cells conditioned medium (HESCM).

Materials and methods: In this experimental study, 240 germinal vesicle (GV) oocytes were took from NMRI female mice, aged 4-6 weeks, 48 hours before injection of 5 IU pregnant mare serum gonadotropin (PMSG). 120 GV oocytes without cumulus cells were cultured in each of the groups. 120 GV were cultured in HESCM as test groups and also 120 GV cultured in human embryonic stem cells medium (HESM) as control groups. After evaluating the metaphase II (MII) oocyte maturation rate at 8, 16 and 24 hours, the MII oocytes subsequently were fertilized in vitro and the two-cell embryo development rate was recorded at days 1, 2, and 3. Statistical analysis was performed by using the generalized estimating equations (GEE) method that calculated their rate ratio.

Results: Our data indicated there are significant differences between the maturation rates in HESCM and HESM (P=0.004), also the two-cell embryo development was significant between two culture media (P=0.00).

Conclusion: Similar to some other studies, the secretome of the HESCM showed a significant impact on the IVM outcomes in mice.

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人胚胎干细胞条件培养基对小鼠卵母细胞体外成熟的影响。

目的:一些报道表明，生长中的小鼠胚胎干细胞(ESCs)的条件培养基为小卵泡生长、卵母细胞成熟和随后的胚胎生长提供了支持条件。本研究的目的是评估使用人胚胎干细胞条件培养基(HESCM)的未成熟小鼠卵母细胞的体外成熟(IVM)和随后的体外受精(IVF)结果。材料与方法:在注射5 IU孕母马血清促性腺激素(PMSG) 48小时前，取4 ~ 6周龄NMRI雌性小鼠240只生发囊泡(GV)卵母细胞。各组培养不含卵丘细胞的GV卵母细胞120个。将120株GV培养于HESCM中作为试验组，将120株GV培养于人胚胎干细胞培养基(HESM)中作为对照组。在8、16和24小时评估中期II (MII)卵母细胞成熟率后，随后将MII卵母细胞体外受精，并在第1、2和3天记录双细胞胚胎发育率。采用广义估计方程(GEE)方法进行统计分析，计算出它们的比率。结果:我们的数据显示，HESCM和HESM的成熟率差异有统计学意义(P=0.004)，两种培养基的双细胞胚胎发育差异有统计学意义(P=0.00)。结论:与其他一些研究类似，HESCM分泌组对小鼠IVM结果有显著影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Journal CELL BIOLOGY-

CiteScore

3.40

自引率

5.00%

发文量

审稿时长

12 months

期刊介绍： The “Cell Journal (Yakhteh)“, formerly published as “Yakhteh Medical Journal”, is a quarterly English publication of Royan Institute. This journal focuses on topics relevant to cellular and molecular scientific areas, besides other related fields. The Cell J has been certified by Ministry of Culture and Islamic Guidance in 1999 and was accredited as a scientific and research journal by HBI (Health and Biomedical Information) Journal Accreditation Commission in 2000 which is an open access journal.