The protective effects of hyperoxic pre-treatment in human-derived adipose tissue mesenchymal stem cells against in vitro oxidative stress and a rat model of renal ischaemia-reperfusion.

IF 2.5 4区医学 Q3 ENDOCRINOLOGY & METABOLISM Archives of Physiology and Biochemistry Pub Date : 2024-12-01 Epub Date: 2023-07-28 DOI:10.1080/13813455.2023.2238918

Shahram Ahmadi Somaghian, Naser Pajouhi, Omid Dezfoulian, Afshin Pirnia, Ayat Kaeidi, Bahram Rasoulian

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Abstract

Objective: Improvement of cell survival is essential for achieving better clinical outcomes in stem cell therapy. We investigated the effects of hyperoxic pre-treatment (HP) on the viability of human adipose stromal stem cells (ASCs).

Materials and methods: MTT and Western blot tests were used to assess cell viability and the expression of apoptosis-related proteins, respectively. For the in-vivo trial, the rats were subjected to renal ischaemia-reperfusion (IR).

Results: The results showed that HP could significantly increase the viability of ASCs and decrease apoptotic markers (Bax/BCL-2 ratio and Caspase-3) compared with control cells. There were some additional effects with regard to the improvement of renal structure and function in the animal model. However, the difference between the treated and non-treated transplanted ASCs failed to reach significance.

Conclusion: These results suggested that HP could increase the survival of ASCs against oxidative stress-induced damages in the in-vitro condition, but this strategy was not highly effective in renal IR.

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高氧预处理对人源性脂肪组织间充质干细胞抗体外氧化应激和肾缺血再灌注大鼠模型的保护作用。

目的:提高细胞存活率是干细胞治疗取得更好临床效果的关键。我们研究了高氧预处理(HP)对人脂肪基质干细胞(ASCs)活力的影响。材料和方法:采用MTT法和Western blot法分别检测细胞活力和凋亡相关蛋白的表达。体内实验采用肾缺血再灌注法(IR)。结果:与对照细胞相比，HP可显著提高ASCs的活力，降低凋亡标志物(Bax/BCL-2比值和Caspase-3)。在改善动物模型肾脏结构和功能方面有一些额外的作用。然而，经治疗和未治疗的移植ASCs之间的差异没有达到显著性。结论:HP可提高体外条件下ASCs抗氧化应激损伤的存活率，但在肾IR中效果不明显。

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来源期刊

Archives of Physiology and Biochemistry ENDOCRINOLOGY & METABOLISM-PHYSIOLOGY

CiteScore

6.90

自引率

3.30%

发文量

期刊介绍： Archives of Physiology and Biochemistry: The Journal of Metabolic Diseases is an international peer-reviewed journal which has been relaunched to meet the increasing demand for integrated publication on molecular, biochemical and cellular aspects of metabolic diseases, as well as clinical and therapeutic strategies for their treatment. It publishes full-length original articles, rapid papers, reviews and mini-reviews on selected topics. It is the overall goal of the journal to disseminate novel approaches to an improved understanding of major metabolic disorders. The scope encompasses all topics related to the molecular and cellular pathophysiology of metabolic diseases like obesity, type 2 diabetes and the metabolic syndrome, and their associated complications. Clinical studies are considered as an integral part of the Journal and should be related to one of the following topics: -Dysregulation of hormone receptors and signal transduction -Contribution of gene variants and gene regulatory processes -Impairment of intermediary metabolism at the cellular level -Secretion and metabolism of peptides and other factors that mediate cellular crosstalk -Therapeutic strategies for managing metabolic diseases Special issues dedicated to topics in the field will be published regularly.