Single-Cell Multiomics.

IF 7 Q1 MATHEMATICAL & COMPUTATIONAL BIOLOGY Annual Review of Biomedical Data Science Pub Date : 2023-08-10 Epub Date: 2023-05-09 DOI:10.1146/annurev-biodatasci-020422-050645
Emily Flynn, Ana Almonte-Loya, Gabriela K Fragiadakis
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引用次数: 0

Abstract

Single-cell RNA sequencing methods have led to improved understanding of the heterogeneity and transcriptomic states present in complex biological systems. Recently, the development of novel single-cell technologies for assaying additional modalities, specifically genomic, epigenomic, proteomic, and spatial data, allows for unprecedented insight into cellular biology. While certain technologies collect multiple measurements from the same cells simultaneously, even when modalities are separately assayed in different cells, we can apply novel computational methods to integrate these data. The application of computational integration methods to multimodal paired and unpaired data results in rich information about the identities of the cells present and the interactions between different levels of biology, such as between genetic variation and transcription. In this review, we both discuss the single-cell technologies for measuring these modalities and describe and characterize a variety of computational integration methods for combining the resulting data to leverage multimodal information toward greater biological insight.

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单细胞多组学
单细胞 RNA 测序方法提高了人们对复杂生物系统中异质性和转录组状态的认识。最近,用于检测其他模式(特别是基因组学、表观基因组学、蛋白质组学和空间数据)的新型单细胞技术的发展,使人们对细胞生物学有了前所未有的深入了解。某些技术能同时从同一个细胞中收集多种测量数据,即使是在不同细胞中分别检测的模式,我们也能应用新型计算方法来整合这些数据。将计算整合方法应用于多模态配对和非配对数据,可获得丰富的信息,包括存在的细胞身份以及不同生物学水平之间的相互作用,如遗传变异和转录之间的相互作用。在这篇综述中,我们既讨论了测量这些模式的单细胞技术,也介绍了各种计算整合方法,并说明了这些方法的特点。
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来源期刊
CiteScore
11.10
自引率
1.70%
发文量
0
期刊介绍: The Annual Review of Biomedical Data Science provides comprehensive expert reviews in biomedical data science, focusing on advanced methods to store, retrieve, analyze, and organize biomedical data and knowledge. The scope of the journal encompasses informatics, computational, artificial intelligence (AI), and statistical approaches to biomedical data, including the sub-fields of bioinformatics, computational biology, biomedical informatics, clinical and clinical research informatics, biostatistics, and imaging informatics. The mission of the journal is to identify both emerging and established areas of biomedical data science, and the leaders in these fields.
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