Enhancement in Bacterial Cellulolytic Enzyme Production Using Acid-Pretreated Banana Peel Waste: A Comparative Evaluation.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biotechnology Pub Date : 2024-08-01 Epub Date: 2023-08-10 DOI:10.1007/s12033-023-00816-w

Pathan Ahemad Khan, Basant Lal, Preeti Singh, Rajeev Singh, Asad Syed, Abdallah M Elgorban, Meenakshi Verma, P K Mishra, Bhawna Verma, Neha Srivastava, Anthonia O'Donovan

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Abstract

Banana peel waste is one of the major contributors in the issue raised from solid waste, however, it can be valorized effectively due to high content of cellulose and hemicellulose. Significant conversion of banana waste includes cellulolytic enzymes and bioenergy production. In the present study, bacterial cellulase was produced using raw banana peel and ripe banana peel under SSF. Additionally, impact of acid pretreatment was investigated as one of strategy to improve cellulolytic enzyme production. A comparative evaluation of raw and ripe banana peels showed that ripe banana peels showed better enzyme production after pretreatment with 0.5% dilute HCl acid. In the series of enhancement of the enzyme production, temperature and pH of the SSF medium were also investigated, and found temperature 35 °C and pH 6.0 were optimum to produce maximum 3.5-U/ml FPA, 39-U/ml BGL, and 54-U/ml EG in 18-h SSF incubation. The study presented eco-friendly waste management to produce industrial enzyme for its promising application in waste valorization and biorefinery area.

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利用经酸性预处理的香蕉皮废料提高细菌纤维素分解酶的产量：比较评估。

香蕉皮废弃物是固体废弃物问题的主要成因之一，但由于其纤维素和半纤维素含量较高，可以有效地加以利用。香蕉废料的重要转化包括纤维素分解酶和生物能源生产。本研究利用生香蕉皮和熟香蕉皮在 SSF 条件下生产细菌纤维素酶。此外，还研究了酸预处理对提高纤维素分解酶产量的影响。对生香蕉皮和熟香蕉皮的比较评估表明，熟香蕉皮在用 0.5% 稀盐酸预处理后，产酶量更高。在一系列提高酶产量的过程中，还对 SSF 培养基的温度和 pH 值进行了研究，结果发现温度 35 ℃ 和 pH 值 6.0 最适合在 18 小时 SSF 培养中产生最多 3.5-U/ml FPA、39-U/ml BGL 和 54-U/ml EG。该研究介绍了利用生态友好型废物管理生产工业酶的方法，其在废物估值和生物炼制领域的应用前景广阔。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.