Characterization of HPV subtypes in invasive cervical cancer in Botswana patients using a pan-pathogen microarray technology

IF 4.7 Q1 VIROLOGY Tumour Virus Research Pub Date : 2023-06-01 DOI:10.1016/j.tvr.2023.200262
Surbhi Grover , Tyler Seckar , Le Gao , Rohini Bhatia , Xiang Lin , Nicola Zetola , Doreen Ramogola-Masire , Erle Robertson
{"title":"Characterization of HPV subtypes in invasive cervical cancer in Botswana patients using a pan-pathogen microarray technology","authors":"Surbhi Grover ,&nbsp;Tyler Seckar ,&nbsp;Le Gao ,&nbsp;Rohini Bhatia ,&nbsp;Xiang Lin ,&nbsp;Nicola Zetola ,&nbsp;Doreen Ramogola-Masire ,&nbsp;Erle Robertson","doi":"10.1016/j.tvr.2023.200262","DOIUrl":null,"url":null,"abstract":"<div><p>Human papillomavirus (HPV) plays a significant role in the development of cervical cancers in the setting of co-infection with HIV. Botswana has a high prevalence of HIV and cervical cancer. In this study, we investigated the distribution of HPV subtypes in cervical cancer biopsy samples from patients in Botswana using a highly sensitive pan-pathogen microarray technology, PathoChip, to detect both high- (HR-HPV) and low-risk HPV (LR-HPV) subtypes in women living with HIV (WLWH) and women living without HIV. We analyzed samples from 168 patients, of which 73% (n = 123) were WLWH with a median CD4 count of 479.5 cells/μL. Five HR-HPV subtypes were detected in the cohort: HPV 16, 18, 26, 34, and 53. The most prevalent subtypes were HPV 26 (96%) and HPV 34 (92%); 86% of WLWH (n = 106) had co-infection with four or more HR-HPV subtypes compared to 67% (n = 30) of women without HIV (p &lt; 0.01). We detected 66 LR-HPV subtypes among all cervical cancer patients, with HPV 6b and 48 being most prevalent. Notably, signatures for LR-HPV subtypes 10, 41, 90, and 129 were only detected in WLWH. Signal intensity for HPV 18 was significantly weaker in WLWH with CD4 levels ≤200 cells/μL as compared to patients with &gt;200 cells/μL and HIV-negative patients. Although the majority of cervical cancer specimens in this cohort were determined to have multiple HPV infections, the most prevalent HR-HPV subtypes (HPV 26 and HPV34) found in these cervical cancer samples are not covered in the current HPV vaccines. Though no conclusions can be made on the direct carcinogenicity of these subtypes the results do underlie the need for continued screening for prevention of cervical cancer.</p></div>","PeriodicalId":52381,"journal":{"name":"Tumour Virus Research","volume":"15 ","pages":"Article 200262"},"PeriodicalIF":4.7000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1a/dc/main.PMC10239018.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tumour Virus Research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666679023000095","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VIROLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Human papillomavirus (HPV) plays a significant role in the development of cervical cancers in the setting of co-infection with HIV. Botswana has a high prevalence of HIV and cervical cancer. In this study, we investigated the distribution of HPV subtypes in cervical cancer biopsy samples from patients in Botswana using a highly sensitive pan-pathogen microarray technology, PathoChip, to detect both high- (HR-HPV) and low-risk HPV (LR-HPV) subtypes in women living with HIV (WLWH) and women living without HIV. We analyzed samples from 168 patients, of which 73% (n = 123) were WLWH with a median CD4 count of 479.5 cells/μL. Five HR-HPV subtypes were detected in the cohort: HPV 16, 18, 26, 34, and 53. The most prevalent subtypes were HPV 26 (96%) and HPV 34 (92%); 86% of WLWH (n = 106) had co-infection with four or more HR-HPV subtypes compared to 67% (n = 30) of women without HIV (p < 0.01). We detected 66 LR-HPV subtypes among all cervical cancer patients, with HPV 6b and 48 being most prevalent. Notably, signatures for LR-HPV subtypes 10, 41, 90, and 129 were only detected in WLWH. Signal intensity for HPV 18 was significantly weaker in WLWH with CD4 levels ≤200 cells/μL as compared to patients with >200 cells/μL and HIV-negative patients. Although the majority of cervical cancer specimens in this cohort were determined to have multiple HPV infections, the most prevalent HR-HPV subtypes (HPV 26 and HPV34) found in these cervical cancer samples are not covered in the current HPV vaccines. Though no conclusions can be made on the direct carcinogenicity of these subtypes the results do underlie the need for continued screening for prevention of cervical cancer.

Abstract Image

Abstract Image

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
使用泛病原体微阵列技术表征博茨瓦纳患者侵袭性宫颈癌的HPV亚型
人乳头瘤病毒(HPV)在与HIV共同感染的情况下,在宫颈癌的发展中起着重要作用。博茨瓦纳艾滋病毒和癌症的发病率很高。在这项研究中,我们使用高度敏感的全抗原微阵列技术PathoChip,对博茨瓦纳癌症宫颈活检样本中HPV亚型的分布进行了调查,以检测艾滋病毒感染者(WLWH)和无艾滋病毒感染者中的高风险(HR-HPV)和低风险HPV(LR-HPV)亚型。我们分析了168名患者的样本,其中73%(n=123)为WLWH,CD4计数中位数为479.5个细胞/μL。在队列中检测到五种HR-HPV亚型:HPV 16、18、26、34和53。最常见的亚型是HPV 26(96%)和HPV 34(92%);86%的WLWH(n=106)与四种或四种以上的HR-HPV亚型共感染,相比之下,67%(n=30)的无HIV的妇女(p<0.01)。值得注意的是,LR-HPV亚型10、41、90和129的特征仅在WLWH中检测到。CD4水平≤200个细胞/μ;μL和HIV阴性患者。尽管该队列中的大多数癌症标本被确定为具有多种HPV感染,但在这些癌症宫颈样本中发现的最流行的HR-HPV亚型(HPV 26和HPV34)不包括在当前的HPV疫苗中。虽然还不能对这些亚型的直接致癌性做出结论,但这些结果确实是继续筛查预防宫颈癌症的必要性的基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Tumour Virus Research
Tumour Virus Research Medicine-Infectious Diseases
CiteScore
6.50
自引率
2.30%
发文量
16
审稿时长
56 days
期刊最新文献
JC Virus Small Tumor Antigen Promotes S Phase Entry and Cell Cycle Progression. Modulation of epithelial homeostasis by HPV using Notch and Wnt Modulation of connexin 43 in viral infections The imprint of viral oncoproteins on the variable clinical behavior among human papilloma virus-related oropharyngeal squamous cell carcinomas Genomic diversity of HPV6 and HPV11 in recurrent respiratory papillomatosis: Association with malignant transformation in the lungs and clinical outcomes
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1