An inhibitory mechanism for suppressing high salt intake in Drosophila.

IF 2.8 4区 心理学 Q1 BEHAVIORAL SCIENCES Chemical Senses Pub Date : 2023-01-01 DOI:10.1093/chemse/bjad014
Manali Dey, Anindya Ganguly, Anupama Dahanukar
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Abstract

High concentrations of dietary salt are harmful to health. Like most animals, Drosophila melanogaster are attracted to foods that have low concentrations of salt, but show strong taste avoidance of high salt foods. Salt in known on multiple classes of taste neurons, activating Gr64f sweet-sensing neurons that drive food acceptance and 2 others (Gr66a bitter and Ppk23 high salt) that drive food rejection. Here we find that NaCl elicits a bimodal dose-dependent response in Gr64f taste neurons, which show high activity with low salt and depressed activity with high salt. High salt also inhibits the sugar response of Gr64f neurons, and this action is independent of the neuron's taste response to salt. Consistent with the electrophysiological analysis, feeding suppression in the presence of salt correlates with inhibition of Gr64f neuron activity, and remains if high salt taste neurons are genetically silenced. Other salts such as Na2SO4, KCl, MgSO4, CaCl2, and FeCl3 act on sugar response and feeding behavior in the same way. A comparison of the effects of various salts suggests that inhibition is dictated by the cationic moiety rather than the anionic component of the salt. Notably, high salt-dependent inhibition is not observed in Gr66a neurons-response to a canonical bitter tastant, denatonium, is not altered by high salt. Overall, this study characterizes a mechanism in appetitive Gr64f neurons that can deter ingestion of potentially harmful salts.

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果蝇抑制高盐摄入的抑制机制
高浓度的膳食盐对健康有害。与大多数动物一样,黑腹果蝇也会被低浓度盐分的食物所吸引,但对高盐分食物则表现出强烈的味觉回避。盐对多种味觉神经元的影响是已知的,它能激活驱动接受食物的 Gr64f 甜味感觉神经元和驱动拒绝食物的另外两种神经元(Gr66a 苦味神经元和 Ppk23 高盐神经元)。在这里,我们发现氯化钠会在 Gr64f 味觉神经元中引起双峰剂量依赖性反应,低盐时活性高,高盐时活性低。高盐也会抑制 Gr64f 神经元的糖反应,而且这种作用与神经元对盐的味觉反应无关。与电生理分析一致的是,食盐存在时的进食抑制与 Gr64f 神经元活性的抑制相关,如果高盐味觉神经元被基因沉默,这种抑制作用仍然存在。其他盐类如 Na2SO4、KCl、MgSO4、CaCl2 和 FeCl3 对糖反应和摄食行为的作用方式相同。对各种盐的作用进行比较后发现,抑制作用是由盐中的阳离子分子而不是阴离子成分决定的。值得注意的是,在 Gr66a 神经元中没有观察到高盐依赖性抑制--对典型苦味剂地那铵的反应不会因高盐而改变。总之,这项研究描述了食欲性 Gr64f 神经元的一种机制,这种机制可以阻止摄入可能有害的盐。
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来源期刊
Chemical Senses
Chemical Senses 医学-行为科学
CiteScore
8.60
自引率
2.90%
发文量
25
审稿时长
1 months
期刊介绍: Chemical Senses publishes original research and review papers on all aspects of chemoreception in both humans and animals. An important part of the journal''s coverage is devoted to techniques and the development and application of new methods for investigating chemoreception and chemosensory structures.
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