Expression and Regulatory Roles of Small Nucleolar RNA Host Gene 4 in Gastric Cancer.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2023-01-01 DOI:10.2174/1389203724666230810094548

Navid Pourghasem, Shadi Ghorbanzadeh, Abdol Azim Nejatizadeh

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Abstract

Aims: The role of SNHG4 in the initiation and development of gastric cancer.

Background: Gastric cancer is one of the leading causes of cancer death worldwide. Studies have shown that lncRNAs have a regulatory function in human diseases, particularly cancers. Small nuclear RNA host gene 4 (SNHG4) has been known as an oncogenic long noncoding RNA (lncRNA) in various cancers, and its dysregulation can lead to tumorigenesis and cancer progression.

Objective: Alteration of SNHG4 expression in gastric cancer and its correlation with clinical features of patients with stomach cancer; also, the accomplishment of bioinformatic analysis to find the potential pathways which could be impressed by changes in SNHG4 RNA expression.

Methods: The present study aims to determine the molecular mechanism of SNHG4 and the effects of its expression on the development of GC. Based on the bioinformatics investigations, we studied gene expression analysis, Kaplan-Meier survival, Gene ontology (GO), KEGG pathway enrichment, microRNA targets, transcription factor targets, and proteins interacting with SNHG4. During the experimental phase, SNHG4 expression was examined by quantitative real-time PCR (qRTPCR) in 40 paired gastric adenocarcinoma tissues and normal neighboring tissues. Also, we investigated the correlation between SNHG4 expression and patients' clinicopathological characteristics.

Results: Increased SNHG4 expression was detected in GC tissues, which is significantly associated with the TNM stage, grade group, tumor size, and metastatic status. Evaluation survival analysis demonstrated that overexpression of SNHG4 in GC tissues is remarkably related to poor overall survival (OS). SNHG4 is closely related to miR-490 and E2F family transcription factors. GO analysis suggested the possible role of SNHG4 in cell-cell adhesion, and KEGG enrichment analysis revealed that SNHG4 could be associated with the gastric cancer signaling pathway. ELAVL1 and IGF2BP2 have the highest number of SNHG4 target sites, and these proteins are involved in the PI3K-Akt-mTOR and ERK-MAPK signaling pathways.

Conclusion: Based on our results, we conclude that SNHG4 may have a function in GC development by regulating tumor-related signaling pathways.

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小核RNA宿主基因4在癌症中的表达及其调控作用。

目的：探讨SNHG4在癌症发生发展中的作用。背景：癌症是全球癌症死亡的主要原因之一。研究表明，lncRNA在人类疾病，特别是癌症中具有调节功能。小核RNA宿主基因4（SNHG4）在多种癌症中被称为致癌的长非编码RNA（lncRNA），其失调可导致肿瘤发生和癌症进展。目的：SNHG4在癌症中的表达变化及其与癌症患者临床特征的相关性；此外，完成了生物信息学分析，以找到SNHG4 RNA表达变化可能影响的潜在途径。方法：本研究旨在确定SNHG4的分子机制及其表达对GC发展的影响。基于生物信息学研究，我们研究了基因表达分析、Kaplan-Meier生存、基因本体论（GO）、KEGG途径富集、微小RNA靶标、转录因子靶标以及与SNHG4相互作用的蛋白质。在实验阶段，通过实时定量PCR（qRTPCR）检测40对胃腺癌组织和正常邻近组织中SNHG4的表达。此外，我们还研究了SNHG4的表达与患者临床病理特征之间的相关性。结果：胃癌组织中SNHG4表达增加，这与TNM分期、分级组、肿瘤大小和转移状态显著相关。评估生存率分析表明，胃癌组织中SNHG4的过度表达与总生存率低（OS）显著相关。SNHG4与miR-490和E2F家族转录因子密切相关。GO分析表明SNHG4可能在细胞间粘附中发挥作用，KEGG富集分析表明，SNHG4与癌症信号通路有关。ELAVL1和IGF2BP2具有最高数量的SNHG4靶位点，并且这些蛋白质参与PI3K-Akt-mTOR和ERK-MAPK信号通路。结论：根据我们的研究结果，我们得出结论，SNHG4可能通过调节肿瘤相关信号通路在GC的发展中发挥作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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