Diclazuril-induced expression of CDK-related kinase 2 in the second-generation merozoites of Eimeria tenella

IF 1.4 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular and biochemical parasitology Pub Date : 2023-09-01 DOI:10.1016/j.molbiopara.2023.111575

Bian-hua Zhou , Hai-yan Ding , Jing-yun Yang , Jun Chai , Hong-wei Guo , Er-jie Tian

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Abstract

Diclazuril is a classic anticoccidial drug. The key molecules of diclazuril in anticoccidial action allows target screening for the development of anticoccidial drugs. Cyclin-dependent kinases (CDK) are prominent target proteins in apicomplexan parasites. In this study, a diclazuril anticoccidiosis animal model was established, and the transcription and translation levels of the CDK-related kinase 2 of Eimeria tenella (EtCRK2) were detected. mRNA and protein expression levels of EtCRK2 decreased in the infected/diclazuril group compared with those in the infected/control group. In addition, immunofluorescence analysis showed that EtCRK2 was localised in the cytoplasm of the merozoites. The fluorescence intensity of EtCRK2 in the infected/diclazuril group was significantly weaker than that in the infected/control group. The anticoccidial drug diclazuril against E.tenella affects the expression pattern of EtCRK2 molecule, and EtCRK2 is a potential target for new drug development.

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双唑脲诱导柔嫩艾美耳球虫第二代裂殖子中CDK相关激酶2的表达。

地拉祖利是一种经典的抗癫痫药物。双氯脲抗球虫作用的关键分子为开发抗球虫药物提供了靶点筛选。细胞周期依赖性激酶（CDK）是顶复门寄生虫的主要靶蛋白。本研究建立了双唑脲抗球虫病动物模型，并检测了柔嫩艾美耳球虫CDK相关激酶2（EtCRK2）的转录和翻译水平。与感染/对照组相比，感染/双氯脲组EtCRK2的mRNA和蛋白表达水平降低。此外，免疫荧光分析显示EtCRK2定位于裂殖子的细胞质中。EtCRK2在感染/双氯脲组中的荧光强度显著弱于感染/对照组。针对E.tenella的抗球虫药物双氯脲影响EtCRK2分子的表达模式，EtCRK2是新药开发的潜在靶点。

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来源期刊

Molecular and biochemical parasitology 医学-寄生虫学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

63 days

期刊介绍： The journal provides a medium for rapid publication of investigations of the molecular biology and biochemistry of parasitic protozoa and helminths and their interactions with both the definitive and intermediate host. The main subject areas covered are: • the structure, biosynthesis, degradation, properties and function of DNA, RNA, proteins, lipids, carbohydrates and small molecular-weight substances • intermediary metabolism and bioenergetics • drug target characterization and the mode of action of antiparasitic drugs • molecular and biochemical aspects of membrane structure and function • host-parasite relationships that focus on the parasite, particularly as related to specific parasite molecules. • analysis of genes and genome structure, function and expression • analysis of variation in parasite populations relevant to genetic exchange, pathogenesis, drug and vaccine target characterization, and drug resistance. • parasite protein trafficking, organelle biogenesis, and cellular structure especially with reference to the roles of specific molecules • parasite programmed cell death, development, and cell division at the molecular level.