A novel approach for direct detection of the IGH::CRLF2 gene fusion by fluorescent in situ hybridization.

IF 1.3 4区生物学 Q4 GENETICS & HEREDITY Molecular Cytogenetics Pub Date : 2023-08-13 DOI:10.1186/s13039-023-00652-2

Rosa María González-Arreola, Adriana García-Romero, María Teresa Magaña-Torres, Juan Ramón González-García

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Abstract

Background: High expression of the Cytokine Receptor-Like Factor 2 (CRLF2) gene has been observed in patients with acute lymphoblastic leukemia BCR-ABL1-like subtype. Currently, there is no commercial system available for the direct detection of the IGH::CRLF2 fusion by fluorescent in situ hybridization (FISH), as there are for many other leukemia-related gene fusions. In an effort to verify the IGH::CRLF2 fusion, some researchers prepare home-grown FISH probes from bacterial artificial chromosome clones flanking the IGH and CRLF2 genes, which is the best alternative to confirm the fusion, however difficult to reproduce in most cytogenetic laboratories.

Results: For the direct observation of the IGH::CRLF2 gene fusion we designed a methodological approach requiring the two commercially available IGH and CRLF2 break-apart probes.

Conclusions: Our methodological approach allows direct visualization of the IGH::CRLF2 gene fusion and has the potential to be used for identification of other gene fusions.

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利用荧光原位杂交直接检测IGH::CRLF2基因融合的新方法。

背景:细胞因子受体样因子2 (CRLF2)基因在急性淋巴细胞白血病bcr - abl1样亚型患者中高表达。目前，还没有商业化的系统可以通过荧光原位杂交(FISH)直接检测IGH::CRLF2融合，因为有许多其他白血病相关的基因融合。为了验证IGH::CRLF2的融合，一些研究人员准备了自制的FISH探针，这些探针来自细菌人工染色体克隆，位于IGH和CRLF2基因的两侧，这是确认融合的最佳选择，但在大多数细胞遗传学实验室很难复制。结果:为了直接观察IGH::CRLF2基因融合，我们设计了一种方法方法，需要两种市售的IGH和CRLF2分离探针。结论:我们的方法可以直接可视化IGH::CRLF2基因融合，并有可能用于鉴定其他基因融合。

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来源期刊

Molecular Cytogenetics GENETICS & HEREDITY-

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： Molecular Cytogenetics encompasses all aspects of chromosome biology and the application of molecular cytogenetic techniques in all areas of biology and medicine, including structural and functional organization of the chromosome and nucleus, genome variation, expression and evolution, chromosome abnormalities and genomic variations in medical genetics and tumor genetics. Molecular Cytogenetics primarily defines a large set of the techniques that operate either with the entire genome or with specific targeted DNA sequences. Topical areas include, but are not limited to: -Structural and functional organization of chromosome and nucleus- Genome variation, expression and evolution- Animal and plant molecular cytogenetics and genomics- Chromosome abnormalities and genomic variations in clinical genetics- Applications in preimplantation, pre- and post-natal diagnosis- Applications in the central nervous system, cancer and haematology research- Previously unreported applications of molecular cytogenetic techniques- Development of new techniques or significant enhancements to established techniques. This journal is a source for numerous scientists all over the world, who wish to improve or introduce molecular cytogenetic techniques into their practice.