Nanoencapsulation and characterisation of Hypericum perforatum for the treatment of neuropathic pain.

IF 3 4区医学 Q2 CHEMISTRY, APPLIED Journal of microencapsulation Pub Date : 2023-09-01 DOI:10.1080/02652048.2023.2215306

Radha Goel, Nitin Kumar, Neelam Singh, Rosaline Mishra

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Abstract

Aim: This work aimed to encapsulate Hypericum perforatum extract (HPE) into nanophytosomes (NPs) and assess the therapeutic efficacy of this nanocarrier in neuropathic pain induced by partial sciatic nerve ligation (PSNL).

Methods: Hydroalcoholic extract of Hypericum perforatum was prepared and encapsulated into NPs by thin layer hydration method. Particle size, zeta potential, TEM, differential scanning calorimetry (DSC), entrapment efficiency (%EE), and loading capacity (LC) of NPs were reported. The biochemical and histopathological examinations were measured in the sciatic nerve.

Results: Particle size, zeta potential, %EE, and LC were 104.7 ± 1.529 nm, -8.93 ± 1.71 mV, 87.23 ± 1.3%, and 53.12 ± 1.7%, respectively. TEM revealed well-formed and distinct vesicles. NPHPE (NPs of HPE) was significantly more effective than HPE in reducing PSNL-inducing pain. Antioxidant levels and sciatic nerve histology were reversed to normal with NPHPE.

Conclusions: This study demonstrates that encapsulating HPE with phytosomes is an effective therapeutic approach for neuropathic pain.

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贯叶连翘治疗神经性疼痛的纳米胶囊化及表征。

目的:将贯叶连翘提取物(HPE)包埋在纳米植物体(NPs)中，观察该纳米载体对部分坐骨神经结扎(PSNL)所致神经性疼痛的治疗效果。方法:制备贯叶连翘水醇提取物，采用薄层水合法制备NPs。报道了NPs的粒径、zeta电位、透射电镜(TEM)、差示扫描量热法(DSC)、包裹效率(%EE)和负载能力(LC)。对坐骨神经进行生化及组织病理学检查。结果:粒径为104.7±1.529 nm, zeta电位为-8.93±1.71 mV， %EE为87.23±1.3%，LC为53.12±1.7%。透射电镜显示形态良好且明显的囊泡。NPHPE (HPE的NPs)在减轻psnl引起的疼痛方面明显优于HPE。NPHPE组抗氧化水平和坐骨神经组织学恢复正常。结论:本研究表明，磷脂体包埋HPE是治疗神经性疼痛的有效方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.