Factors affecting the shear wave elastic quantitative measurement of penile tissue in rats.

IF 0.4 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Asian Biomedicine Pub Date : 2023-02-01 DOI:10.2478/abm-2023-0040

Wan-Ting Rao, Jing-Dong Tang, Jin-Fang Xing

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Abstract

Background: As a new ultrasound technology, 2-dimensional shear wave elastography (2D-SWE) can evaluate the elastic characteristics of penile tissue. However, no studies have reported the main factors affecting the shear wave elastic quantitative measurement (SWQ) in penile tissue.

Objectives: To analyze the main factors affecting the SWQ reflecting the elastic characteristics of penile tissue by 2D-SWE.

Methods: Twenty healthy male Sprague-Dawley rats (5-60 weeks old) were selected for this study. We performed the 2D-SWE examination on the penis using the Aixplorer ultrasound system, with SWQ as the measurement index. We performed penile immunohistochemistry analysis with the positive area proportion (PAP) of alpha-smooth muscle actin (PAP_S) and type III collagen fiber (PAP_C) as the outcomes. Then, we conducted multiple linear regression analysis to explore the correlation of SWQ with PAP_S and PAP_C and established the regression equation.

Results: The multiple linear regression analysis showed that the linear regression equation (SWQ = 10.376 - 0.05 PAP_S - 0.07 PAP_C) was statistically significant (F = 21.153, P < 0.001). The content of smooth muscle cells (SMCs) and collagen fibers was negatively correlated with SWQ, affecting 42.6% of the total variation in SWQ (R² = 0.426).

Conclusions: SMCs and collagen fibers are the main factors affecting the SWQ value of penile tissue and the primary tissue components determining the SWQ when using 2D-SWE to quantitatively evaluate the elastic characteristics of penile tissue.

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影响大鼠阴茎组织剪切波弹性定量测量的因素。

背景:二维横波弹性成像(2D-SWE)作为一种新的超声技术，可以评价阴茎组织的弹性特性。然而，目前还没有研究报道影响阴茎组织剪切波弹性定量测量(SWQ)的主要因素。目的:分析影响2D-SWE反映阴茎组织弹性特性的SWQ的主要因素。方法:选取健康雄性Sprague-Dawley大鼠20只(5 ~ 60周龄)。我们使用Aixplorer超声系统对阴茎进行2D-SWE检查，以SWQ为测量指标。我们以α -平滑肌肌动蛋白(PAPS)和III型胶原纤维(PAPC)阳性面积比例(PAP)作为结果进行阴茎免疫组化分析。然后，我们进行多元线性回归分析，探讨SWQ与PAPS和PAPC的相关性，并建立回归方程。结果:多元线性回归分析显示，线性回归方程(SWQ = 10.376 - 0.05 PAPS - 0.07 PAPC)具有统计学意义(F = 21.153, P < 0.001)。平滑肌细胞(SMCs)和胶原纤维含量与SWQ呈负相关，影响SWQ总变异量的42.6% (R2 = 0.426)。结论:在2D-SWE定量评价阴茎组织弹性特性时，SMCs和胶原纤维是影响阴茎组织SWQ值的主要因素，也是决定阴茎组织SWQ值的主要组织成分。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Asian Biomedicine 医学-医学：研究与实验

CiteScore

1.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Asian Biomedicine: Research, Reviews and News (ISSN 1905-7415 print; 1875-855X online) is published in one volume (of 6 bimonthly issues) a year since 2007. [...]Asian Biomedicine is an international, general medical and biomedical journal that aims to publish original peer-reviewed contributions dealing with various topics in the biomedical and health sciences from basic experimental to clinical aspects. The work and authorship must be strongly affiliated with a country in Asia, or with specific importance and relevance to the Asian region. The Journal will publish reviews, original experimental studies, observational studies, technical and clinical (case) reports, practice guidelines, historical perspectives of Asian biomedicine, clinicopathological conferences, and commentaries Asian biomedicine is intended for a broad and international audience, primarily those in the health professions including researchers, physician practitioners, basic medical scientists, dentists, educators, administrators, those in the assistive professions, such as nurses, and the many types of allied health professionals in research and health care delivery systems including those in training.