用纤维光度法估计小鼠睡眠时荧光生物传感器水平。

Mie Andersen, Anastasia Tsopanidou, Tessa Radovanovic, Viviane Noelani Compere, Natalie Hauglund, Maiken Nedergaard, Celia Kjaerby
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引用次数: 0

摘要

睡眠不是同质的,而是包含高度多样化的受神经调节剂影响的微观结构组成。先前用于测量体内神经调节剂水平的方法受到时间分辨率低或在自由移动环境中实现记录的技术困难的限制,这对自然睡眠至关重要。在这个方案中,我们展示了脑电图(EEG)/肌电图(EMG)记录与荧光生物传感器的纤维光度测量相结合,用于自由运动小鼠的神经调节剂。这允许在睡眠的不同阶段以高时间分辨率实时评估细胞外神经调节剂水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Using Fiber Photometry in Mice to Estimate Fluorescent Biosensor Levels During Sleep.

Sleep is not homogenous but contains a highly diverse microstructural composition influenced by neuromodulators. Prior methods used to measure neuromodulator levels in vivo have been limited by low time resolution or technical difficulties in achieving recordings in a freely moving setting, which is essential for natural sleep. In this protocol, we demonstrate the combination of electroencephalographic (EEG)/electromyographic (EMG) recordings with fiber photometric measurements of fluorescent biosensors for neuromodulators in freely moving mice. This allows for real-time assessment of extracellular neuromodulator levels during distinct phases of sleep with a high temporal resolution.

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