Ulises Garza-Ramos, Jesús Silva-Sánchez, Luis Esaú López-Jácome, Melissa Hernández-Durán, Claudia Adriana Colín-Castro, Alejandro Sánchez-Pérez, Jonathan Rodríguez-Santiago, Rayo Morfín-Otero, Eduardo Rodriguez-Noriega, María-Del-Consuelo Velázquez-Acosta, María Del Rosario Vázquez-Larios, José Manuel Feliciano-Guzmán, Fabián Rojas-Larios, Alfredo Ponce-De-Leon, Margarita Lozano-Garcia, Elena Victoria Choy-Chang, Eduardo López-Gutiérrez, Aarón Molina-Jaimes, Mariana Gil-Veloz, Reyna Edith Corte-Rojas, Ismelda López-Ovilla, Jose Luis Ramirez-Mis, Dora Elia Rodríguez-Balderas, Alejandro Molina-Chavarria, Cecilia Padilla-Ibarra, Maria Angelina Quevedo-Ramos, Christian Daniel Mireles-Dávalos, Nadia Rodríguez-Medina, Daira Rubio-Mendoza, Carlos Córdova-Fletes, Flora Cruz-López, Dilva Angelina Becerra-Montejano, Roberto Mercado-Longoria, Rebeca Thelma Martínez-Villarreal, Nicolás Rogelio Eric Barlandas-Rendón, Juan Pablo Mena-Ramírez, Carlos Antonio Couoh-May, Margarita Alcaraz-Espejel, César Adame-Alvarez, Lourdes Hernández-Vicente, Elvira Garza-González
{"title":"墨西哥COVID-19大流行期间革兰氏阴性菌碳青霉烯酶编码基因和粘菌素耐药性:来自Invifar网络的结果","authors":"Ulises Garza-Ramos, Jesús Silva-Sánchez, Luis Esaú López-Jácome, Melissa Hernández-Durán, Claudia Adriana Colín-Castro, Alejandro Sánchez-Pérez, Jonathan Rodríguez-Santiago, Rayo Morfín-Otero, Eduardo Rodriguez-Noriega, María-Del-Consuelo Velázquez-Acosta, María Del Rosario Vázquez-Larios, José Manuel Feliciano-Guzmán, Fabián Rojas-Larios, Alfredo Ponce-De-Leon, Margarita Lozano-Garcia, Elena Victoria Choy-Chang, Eduardo López-Gutiérrez, Aarón Molina-Jaimes, Mariana Gil-Veloz, Reyna Edith Corte-Rojas, Ismelda López-Ovilla, Jose Luis Ramirez-Mis, Dora Elia Rodríguez-Balderas, Alejandro Molina-Chavarria, Cecilia Padilla-Ibarra, Maria Angelina Quevedo-Ramos, Christian Daniel Mireles-Dávalos, Nadia Rodríguez-Medina, Daira Rubio-Mendoza, Carlos Córdova-Fletes, Flora Cruz-López, Dilva Angelina Becerra-Montejano, Roberto Mercado-Longoria, Rebeca Thelma Martínez-Villarreal, Nicolás Rogelio Eric Barlandas-Rendón, Juan Pablo Mena-Ramírez, Carlos Antonio Couoh-May, Margarita Alcaraz-Espejel, César Adame-Alvarez, Lourdes Hernández-Vicente, Elvira Garza-González","doi":"10.1089/mdr.2022.0226","DOIUrl":null,"url":null,"abstract":"<p><p>In this study, we report the carbapenemase-encoding genes and colistin resistance in <i>Escherichia coli</i>, <i>Klebsiella pneumoniae</i>, <i>Acinetobacter baumannii</i>, and <i>Pseudomonas aeruginosa</i> in the second year of the COVID-19 pandemic. Clinical isolates included carbapenem-resistant <i>K. pneumoniae</i>, carbapenem-resistant <i>E. coli</i>, carbapenem-resistant <i>A. baumannii</i>, and carbapenem-resistant <i>P. aeruginosa</i>. Carbapenemase-encoding genes were detected by PCR. Carbapenem-resistant <i>K. pneumoniae</i> and carbapenem-resistant <i>E. coli</i> isolates were analyzed using the Rapid Polymyxin NP assay. <i>mcr</i> genes were screened by PCR. Pulsed-field gel electrophoresis and whole-genome sequencing were performed on representative isolates. A total of 80 carbapenem-resistant <i>E. coli</i>, 103 carbapenem-resistant <i>K. pneumoniae</i>, 284 carbapenem-resistant <i>A. baumannii</i>, and 129 carbapenem-resistant <i>P. aeruginosa</i> isolates were recovered. All carbapenem-resistant <i>E. coli</i> and carbapenem-resistant <i>K. pneumoniae</i> isolates were included for further analysis. A selection of carbapenem-resistant <i>A. baumannii</i> and carbapenem-resistant <i>P. aeruginosa</i> strains was further analyzed (86 carbapenem-resistant <i>A. baumannii</i> and 82 carbapenem-resistant <i>P. aeruginosa</i>). Among carbapenem-resistant <i>K. pneumoniae</i> and carbapenem-resistant <i>E. coli</i> isolates, the most frequent gene was <i>bla</i><sub>NDM</sub> (86/103 [83.5%] and 72/80 [90%], respectively). For carbapenem-resistant <i>A. baumannii</i>, the most frequently detected gene was <i>bla</i><sub>OXA-40</sub> (52/86, 60.5%), and for carbapenem-resistant <i>P. aeruginosa</i>, was <i>bla</i><sub>VIM</sub> (19/82, 23.2%). For carbapenem-resistant <i>A. baumannii</i>, five indistinguishable pulsotypes were detected. Circulation of <i>K. pneumoniae</i> New Delhi metallo-β-lactamase (NDM) and <i>E. coli</i> NDM was detected in Mexico. High virulence sequence types (STs), such as <i>K. pneumoniae</i> ST307, <i>E. coli</i> ST167, <i>P. aeruginosa</i> ST111, and <i>A. baumannii</i> ST2, were detected. Among <i>K. pneumoniae</i> isolates, 18/101 (17.8%) were positive for the Polymyxin NP test (two, 11.0% positive for the <i>mcr-1</i> gene, and one, 5.6% with disruption of the <i>mgrB</i> gene). All <i>E. coli</i> isolates were negative for the Polymyxin NP test. In conclusion, <i>K. pneumoniae</i> NDM and <i>E. coli</i> NDM were detected in Mexico, with the circulation of highly virulent STs. These results are relevant in clinical practice to guide antibiotic therapies considering the molecular mechanisms of resistance to carbapenems.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":"29 6","pages":"239-248"},"PeriodicalIF":2.3000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Carbapenemase-Encoding Genes and Colistin Resistance in Gram-Negative Bacteria During the COVID-19 Pandemic in Mexico: Results from the Invifar Network.\",\"authors\":\"Ulises Garza-Ramos, Jesús Silva-Sánchez, Luis Esaú López-Jácome, Melissa Hernández-Durán, Claudia Adriana Colín-Castro, Alejandro Sánchez-Pérez, Jonathan Rodríguez-Santiago, Rayo Morfín-Otero, Eduardo Rodriguez-Noriega, María-Del-Consuelo Velázquez-Acosta, María Del Rosario Vázquez-Larios, José Manuel Feliciano-Guzmán, Fabián Rojas-Larios, Alfredo Ponce-De-Leon, Margarita Lozano-Garcia, Elena Victoria Choy-Chang, Eduardo López-Gutiérrez, Aarón Molina-Jaimes, Mariana Gil-Veloz, Reyna Edith Corte-Rojas, Ismelda López-Ovilla, Jose Luis Ramirez-Mis, Dora Elia Rodríguez-Balderas, Alejandro Molina-Chavarria, Cecilia Padilla-Ibarra, Maria Angelina Quevedo-Ramos, Christian Daniel Mireles-Dávalos, Nadia Rodríguez-Medina, Daira Rubio-Mendoza, Carlos Córdova-Fletes, Flora Cruz-López, Dilva Angelina Becerra-Montejano, Roberto Mercado-Longoria, Rebeca Thelma Martínez-Villarreal, Nicolás Rogelio Eric Barlandas-Rendón, Juan Pablo Mena-Ramírez, Carlos Antonio Couoh-May, Margarita Alcaraz-Espejel, César Adame-Alvarez, Lourdes Hernández-Vicente, Elvira Garza-González\",\"doi\":\"10.1089/mdr.2022.0226\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In this study, we report the carbapenemase-encoding genes and colistin resistance in <i>Escherichia coli</i>, <i>Klebsiella pneumoniae</i>, <i>Acinetobacter baumannii</i>, and <i>Pseudomonas aeruginosa</i> in the second year of the COVID-19 pandemic. Clinical isolates included carbapenem-resistant <i>K. pneumoniae</i>, carbapenem-resistant <i>E. coli</i>, carbapenem-resistant <i>A. baumannii</i>, and carbapenem-resistant <i>P. aeruginosa</i>. Carbapenemase-encoding genes were detected by PCR. Carbapenem-resistant <i>K. pneumoniae</i> and carbapenem-resistant <i>E. coli</i> isolates were analyzed using the Rapid Polymyxin NP assay. <i>mcr</i> genes were screened by PCR. Pulsed-field gel electrophoresis and whole-genome sequencing were performed on representative isolates. A total of 80 carbapenem-resistant <i>E. coli</i>, 103 carbapenem-resistant <i>K. pneumoniae</i>, 284 carbapenem-resistant <i>A. baumannii</i>, and 129 carbapenem-resistant <i>P. aeruginosa</i> isolates were recovered. All carbapenem-resistant <i>E. coli</i> and carbapenem-resistant <i>K. pneumoniae</i> isolates were included for further analysis. A selection of carbapenem-resistant <i>A. baumannii</i> and carbapenem-resistant <i>P. aeruginosa</i> strains was further analyzed (86 carbapenem-resistant <i>A. baumannii</i> and 82 carbapenem-resistant <i>P. aeruginosa</i>). Among carbapenem-resistant <i>K. pneumoniae</i> and carbapenem-resistant <i>E. coli</i> isolates, the most frequent gene was <i>bla</i><sub>NDM</sub> (86/103 [83.5%] and 72/80 [90%], respectively). For carbapenem-resistant <i>A. baumannii</i>, the most frequently detected gene was <i>bla</i><sub>OXA-40</sub> (52/86, 60.5%), and for carbapenem-resistant <i>P. aeruginosa</i>, was <i>bla</i><sub>VIM</sub> (19/82, 23.2%). For carbapenem-resistant <i>A. baumannii</i>, five indistinguishable pulsotypes were detected. Circulation of <i>K. pneumoniae</i> New Delhi metallo-β-lactamase (NDM) and <i>E. coli</i> NDM was detected in Mexico. High virulence sequence types (STs), such as <i>K. pneumoniae</i> ST307, <i>E. coli</i> ST167, <i>P. aeruginosa</i> ST111, and <i>A. baumannii</i> ST2, were detected. Among <i>K. pneumoniae</i> isolates, 18/101 (17.8%) were positive for the Polymyxin NP test (two, 11.0% positive for the <i>mcr-1</i> gene, and one, 5.6% with disruption of the <i>mgrB</i> gene). All <i>E. coli</i> isolates were negative for the Polymyxin NP test. In conclusion, <i>K. pneumoniae</i> NDM and <i>E. coli</i> NDM were detected in Mexico, with the circulation of highly virulent STs. These results are relevant in clinical practice to guide antibiotic therapies considering the molecular mechanisms of resistance to carbapenems.</p>\",\"PeriodicalId\":18701,\"journal\":{\"name\":\"Microbial drug resistance\",\"volume\":\"29 6\",\"pages\":\"239-248\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2023-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbial drug resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1089/mdr.2022.0226\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbial drug resistance","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1089/mdr.2022.0226","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Carbapenemase-Encoding Genes and Colistin Resistance in Gram-Negative Bacteria During the COVID-19 Pandemic in Mexico: Results from the Invifar Network.
In this study, we report the carbapenemase-encoding genes and colistin resistance in Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa in the second year of the COVID-19 pandemic. Clinical isolates included carbapenem-resistant K. pneumoniae, carbapenem-resistant E. coli, carbapenem-resistant A. baumannii, and carbapenem-resistant P. aeruginosa. Carbapenemase-encoding genes were detected by PCR. Carbapenem-resistant K. pneumoniae and carbapenem-resistant E. coli isolates were analyzed using the Rapid Polymyxin NP assay. mcr genes were screened by PCR. Pulsed-field gel electrophoresis and whole-genome sequencing were performed on representative isolates. A total of 80 carbapenem-resistant E. coli, 103 carbapenem-resistant K. pneumoniae, 284 carbapenem-resistant A. baumannii, and 129 carbapenem-resistant P. aeruginosa isolates were recovered. All carbapenem-resistant E. coli and carbapenem-resistant K. pneumoniae isolates were included for further analysis. A selection of carbapenem-resistant A. baumannii and carbapenem-resistant P. aeruginosa strains was further analyzed (86 carbapenem-resistant A. baumannii and 82 carbapenem-resistant P. aeruginosa). Among carbapenem-resistant K. pneumoniae and carbapenem-resistant E. coli isolates, the most frequent gene was blaNDM (86/103 [83.5%] and 72/80 [90%], respectively). For carbapenem-resistant A. baumannii, the most frequently detected gene was blaOXA-40 (52/86, 60.5%), and for carbapenem-resistant P. aeruginosa, was blaVIM (19/82, 23.2%). For carbapenem-resistant A. baumannii, five indistinguishable pulsotypes were detected. Circulation of K. pneumoniae New Delhi metallo-β-lactamase (NDM) and E. coli NDM was detected in Mexico. High virulence sequence types (STs), such as K. pneumoniae ST307, E. coli ST167, P. aeruginosa ST111, and A. baumannii ST2, were detected. Among K. pneumoniae isolates, 18/101 (17.8%) were positive for the Polymyxin NP test (two, 11.0% positive for the mcr-1 gene, and one, 5.6% with disruption of the mgrB gene). All E. coli isolates were negative for the Polymyxin NP test. In conclusion, K. pneumoniae NDM and E. coli NDM were detected in Mexico, with the circulation of highly virulent STs. These results are relevant in clinical practice to guide antibiotic therapies considering the molecular mechanisms of resistance to carbapenems.
期刊介绍：
Microbial Drug Resistance (MDR) is an international, peer-reviewed journal that covers the global spread and threat of multi-drug resistant clones of major pathogens that are widely documented in hospitals and the scientific community. The Journal addresses the serious challenges of trying to decipher the molecular mechanisms of drug resistance. MDR provides a multidisciplinary forum for peer-reviewed original publications as well as topical reviews and special reports.
MDR coverage includes:
Molecular biology of resistance mechanisms
Virulence genes and disease
Molecular epidemiology
Drug design
Infection control.
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