不明原因流产中催乳素水平影响下子宫内膜CSF-1和CSF-1R的基因表达水平:一项病例对照研究。

IF 2.3 Q2 OBSTETRICS & GYNECOLOGY International Journal of Fertility & Sterility Pub Date : 2023-08-07 DOI:10.22074/ijfs.2023.555588.1316
Zahra Sadeghi Jam, Zahra Tahmasebi Fard
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引用次数: 0

摘要

背景:催乳素等激素通过影响子宫内膜基因的表达,在胚胎植入和发育中发挥着关键作用。本研究旨在评估不孕妇女和复发性流产妇女在体外受精(IVF)妊娠期间血清泌乳素水平及其对子宫内膜组织中集落刺激因子-1(CSF-1)和集落刺激因素-1受体(CSF-1R)基因表达水平的影响,材料和方法:在这项病例对照研究中,40名不孕妇女、40名患有RPL的IVF孕妇和40名因CSF-1和CSF-1R基因而流产的育龄妇女在子宫内膜组织中进行了检测,结果:不孕组平均泌乳素水平为24.38±1.43ng/mL,与可育组(PCSF-1和CSF-1R)有统计学意义,除不孕组外,差异均有统计学意义。结论:本研究显示CSF-1和CSF-1R的表达水平与妊娠损失有显著关系。衰老、肥胖、吸烟和糖尿病等危险因素降低了这两种基因的表达水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Gene Expression Levels of CSF-1 and CSF-1R Endometrial under The Influence of Prolactin Level in Unexplained Miscarriage: A Case-Control Study.

Background: Hormones such as prolactin, by influencing expression of the endometrial genes, play a pivotal role in embryo implantation and development. The present study aimed to evaluate serum level of prolactin and its effect on altering expression level of colony-stimulating factor-1 (CSF-1) and colony-stimulating factor-1 receptor (CSF-1R) genes in endometrial tissue during in vitro fertilization (IVF) pregnancy in the infertile women and recurrent pregnancy loss (RPL), compared to fertile women, who lost their pregnancies at gestational age <20 weeks.

Materials and methods: In this case-control study, 40 infertile women, 40 IVF pregnant women with RPL and 40 fertile women who lost their pregnancies at <20 weeks of gestation for unknown reasons were selected. Prolactin serum level was assessed using ELISA technique and expression of CSF-1 and CSF-1R genes was determined in endometrial tissue, using quantitative reverse transcription polymerase chain reaction (qRT-PCR).

Results: Mean prolactin level of the infertile group was 24.38 ± 1.43 ng/mL and it had statistically significant relationship with the fertile group (P<0.001). Expression level of the CSF-1 and CSF-1R genes were higher in the fertile than infertile groups by 2.88 times (P<0.0001) and 2.64 times (P<0.0001), while it was respectively 2.28 (P<0.0001) and 1.69 (P<0.0001) times higher compared to the RPL group. Risk factors for pregnancy loss, such as aging, increased body mass index (BMI), smoking and diabetes caused decreasing changes in gene expression (CSF-1 and CSF-1R ) and the differences were statistically significant, except in the infertile group.

Conclusion: The present study showed a significant relationship of CSF-1 and CSF-1R expression levels with pregnancy loss. Risk factors such as aging, obesity, smoking and diabetes decreased both genes expression levels.

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来源期刊
CiteScore
4.20
自引率
0.00%
发文量
68
审稿时长
>12 weeks
期刊介绍: International Journal of Fertility & Sterility is a quarterly English publication of Royan Institute . The aim of the journal is to disseminate information through publishing the most recent scientific research studies on Fertility and Sterility and other related topics. Int J Fertil Steril has been certified by Ministry of Culture and Islamic Guidance in 2007 and was accredited as a scientific and research journal by HBI (Health and Biomedical Information) Journal Accreditation Commission in 2008. Int J Fertil Steril is an Open Access journal.
期刊最新文献
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