{"title":"不明原因流产中催乳素水平影响下子宫内膜CSF-1和CSF-1R的基因表达水平:一项病例对照研究。","authors":"Zahra Sadeghi Jam, Zahra Tahmasebi Fard","doi":"10.22074/ijfs.2023.555588.1316","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Hormones such as prolactin, by influencing expression of the endometrial genes, play a pivotal role in embryo implantation and development. The present study aimed to evaluate serum level of prolactin and its effect on altering expression level of colony-stimulating factor-1 (<i>CSF-1</i>) and colony-stimulating factor-1 receptor (<i>CSF-1R</i>) genes in endometrial tissue during <i>in vitro</i> fertilization (IVF) pregnancy in the infertile women and recurrent pregnancy loss (RPL), compared to fertile women, who lost their pregnancies at gestational age <20 weeks.</p><p><strong>Materials and methods: </strong>In this case-control study, 40 infertile women, 40 IVF pregnant women with RPL and 40 fertile women who lost their pregnancies at <20 weeks of gestation for unknown reasons were selected. Prolactin serum level was assessed using ELISA technique and expression of <i>CSF-1</i> and <i>CSF-1R</i> genes was determined in endometrial tissue, using quantitative reverse transcription polymerase chain reaction (qRT-PCR).</p><p><strong>Results: </strong>Mean prolactin level of the infertile group was 24.38 ± 1.43 ng/mL and it had statistically significant relationship with the fertile group (P<0.001). Expression level of the CSF-1 and CSF-1R genes were higher in the fertile than infertile groups by 2.88 times (P<0.0001) and 2.64 times (P<0.0001), while it was respectively 2.28 (P<0.0001) and 1.69 (P<0.0001) times higher compared to the RPL group. Risk factors for pregnancy loss, such as aging, increased body mass index (BMI), smoking and diabetes caused decreasing changes in gene expression (<i>CSF-1</i> and <i>CSF-1R</i> ) and the differences were statistically significant, except in the infertile group.</p><p><strong>Conclusion: </strong>The present study showed a significant relationship of <i>CSF-1</i> and <i>CSF-1R</i> expression levels with pregnancy loss. Risk factors such as aging, obesity, smoking and diabetes decreased both genes expression levels.</p>","PeriodicalId":14080,"journal":{"name":"International Journal of Fertility & Sterility","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cc/a6/Int-J-Fertil-Steril-17-236.PMC10439994.pdf","citationCount":"0","resultStr":"{\"title\":\"Gene Expression Levels of <i>CSF-1</i> and <i>CSF-1R</i> Endometrial under The Influence of Prolactin Level in Unexplained Miscarriage: A Case-Control Study.\",\"authors\":\"Zahra Sadeghi Jam, Zahra Tahmasebi Fard\",\"doi\":\"10.22074/ijfs.2023.555588.1316\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Hormones such as prolactin, by influencing expression of the endometrial genes, play a pivotal role in embryo implantation and development. The present study aimed to evaluate serum level of prolactin and its effect on altering expression level of colony-stimulating factor-1 (<i>CSF-1</i>) and colony-stimulating factor-1 receptor (<i>CSF-1R</i>) genes in endometrial tissue during <i>in vitro</i> fertilization (IVF) pregnancy in the infertile women and recurrent pregnancy loss (RPL), compared to fertile women, who lost their pregnancies at gestational age <20 weeks.</p><p><strong>Materials and methods: </strong>In this case-control study, 40 infertile women, 40 IVF pregnant women with RPL and 40 fertile women who lost their pregnancies at <20 weeks of gestation for unknown reasons were selected. Prolactin serum level was assessed using ELISA technique and expression of <i>CSF-1</i> and <i>CSF-1R</i> genes was determined in endometrial tissue, using quantitative reverse transcription polymerase chain reaction (qRT-PCR).</p><p><strong>Results: </strong>Mean prolactin level of the infertile group was 24.38 ± 1.43 ng/mL and it had statistically significant relationship with the fertile group (P<0.001). Expression level of the CSF-1 and CSF-1R genes were higher in the fertile than infertile groups by 2.88 times (P<0.0001) and 2.64 times (P<0.0001), while it was respectively 2.28 (P<0.0001) and 1.69 (P<0.0001) times higher compared to the RPL group. Risk factors for pregnancy loss, such as aging, increased body mass index (BMI), smoking and diabetes caused decreasing changes in gene expression (<i>CSF-1</i> and <i>CSF-1R</i> ) and the differences were statistically significant, except in the infertile group.</p><p><strong>Conclusion: </strong>The present study showed a significant relationship of <i>CSF-1</i> and <i>CSF-1R</i> expression levels with pregnancy loss. Risk factors such as aging, obesity, smoking and diabetes decreased both genes expression levels.</p>\",\"PeriodicalId\":14080,\"journal\":{\"name\":\"International Journal of Fertility & Sterility\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2023-08-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cc/a6/Int-J-Fertil-Steril-17-236.PMC10439994.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Fertility & Sterility\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22074/ijfs.2023.555588.1316\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"OBSTETRICS & GYNECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Fertility & Sterility","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22074/ijfs.2023.555588.1316","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}
Gene Expression Levels of CSF-1 and CSF-1R Endometrial under The Influence of Prolactin Level in Unexplained Miscarriage: A Case-Control Study.
Background: Hormones such as prolactin, by influencing expression of the endometrial genes, play a pivotal role in embryo implantation and development. The present study aimed to evaluate serum level of prolactin and its effect on altering expression level of colony-stimulating factor-1 (CSF-1) and colony-stimulating factor-1 receptor (CSF-1R) genes in endometrial tissue during in vitro fertilization (IVF) pregnancy in the infertile women and recurrent pregnancy loss (RPL), compared to fertile women, who lost their pregnancies at gestational age <20 weeks.
Materials and methods: In this case-control study, 40 infertile women, 40 IVF pregnant women with RPL and 40 fertile women who lost their pregnancies at <20 weeks of gestation for unknown reasons were selected. Prolactin serum level was assessed using ELISA technique and expression of CSF-1 and CSF-1R genes was determined in endometrial tissue, using quantitative reverse transcription polymerase chain reaction (qRT-PCR).
Results: Mean prolactin level of the infertile group was 24.38 ± 1.43 ng/mL and it had statistically significant relationship with the fertile group (P<0.001). Expression level of the CSF-1 and CSF-1R genes were higher in the fertile than infertile groups by 2.88 times (P<0.0001) and 2.64 times (P<0.0001), while it was respectively 2.28 (P<0.0001) and 1.69 (P<0.0001) times higher compared to the RPL group. Risk factors for pregnancy loss, such as aging, increased body mass index (BMI), smoking and diabetes caused decreasing changes in gene expression (CSF-1 and CSF-1R ) and the differences were statistically significant, except in the infertile group.
Conclusion: The present study showed a significant relationship of CSF-1 and CSF-1R expression levels with pregnancy loss. Risk factors such as aging, obesity, smoking and diabetes decreased both genes expression levels.
期刊介绍:
International Journal of Fertility & Sterility is a quarterly English publication of Royan Institute . The aim of the journal is to disseminate information through publishing the most recent scientific research studies on Fertility and Sterility and other related topics. Int J Fertil Steril has been certified by Ministry of Culture and Islamic Guidance in 2007 and was accredited as a scientific and research journal by HBI (Health and Biomedical Information) Journal Accreditation Commission in 2008. Int J Fertil Steril is an Open Access journal.