系统评估六价铬对男性生育能力的损害和褪黑激素的预防作用:从翻译角度的纵向研究。

IF 3.6 2区 医学 Q2 DEVELOPMENTAL BIOLOGY Molecular human reproduction Pub Date : 2023-06-30 DOI:10.1093/molehr/gaad020
Tianjiao Li, Yinghua Lv, Zhili Wu, Ming Guo, Ruifang Liu, Wenxian Zeng, Yi Zheng
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引用次数: 0

摘要

铬及其化合物与人们的生活密切相关,在工业上有着广泛的应用。过量暴露于六价铬(Cr(VI))会引起包括睾丸在内的多个器官的氧化损伤,对男性生殖健康造成严重威胁。作为一种内源性抗氧化剂,褪黑素具有有效的抗氧化和抗炎特性,成为治疗包括生殖障碍在内的多种疾病的潜在候选者。在这里,通过使用小鼠模型,我们系统地评估了Cr(VI)对男性生殖能力的损害以及褪黑激素的预防作用。我们在腹腔注射Cr(VI)和/或褪黑激素14天后,分析了小鼠睾丸和附睾的组织学和病理学,尾侧附睾精子的密度、活力和畸形,各种生精亚型和Sertoli细胞的增殖活性和凋亡,以及一个精子发生周期内5个时间点(0、14、21、28和35天)小鼠的生育能力。我们发现,Cr(VI)引起的睾丸损伤持续到给药后第21天,然后开始减轻,第35天明显减轻。褪黑素预处理明显减少了Cr(VI)诱导的睾丸损伤,加速了生精恢复,在第35天产生了几乎正常的表型。褪黑素预处理在所有时间点都能保持精子质量。此外,褪黑素在一定程度上保留了Cr(VI)处理小鼠的生育能力,没有明显的副作用。研究结果为褪黑激素作为环境重金属中毒所致男性低生育能力或不育症的治疗药物的临床应用提供了启示。
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Systematic assessment of hexavalent chromium-induced damage to male fertility and the preventive role of melatonin: a longitudinal study from the translational point of view.

Chromium (Cr) and its compounds are closely associated with individuals' lives and extensively used in industry. Excessive exposure to hexavalent chromium (Cr(VI)) induces oxidative damage of various organs including the testes, posing a serious threat to male reproductive fitness. As an endogenous antioxidant, melatonin holds potent antioxidative and anti-inflammatory properties, becoming a potential candidate for treatment of a variety of diseases, including reproductive disorders. Here, by using a mouse model, we systematically assessed Cr(VI)-induced damage to male fertility as well as the preventive role of melatonin. We analyzed the histology and pathology of the testis and epididymis, the density, viability, and malformation of caudal epididymal sperm, the proliferative activity and apoptosis of various spermatogenic subtypes and Sertoli cells, as well as the fertility of mice at five timepoints within one cycle of spermatogenesis (Days 0, 14, 21, 28, and 35) post 14 days of Cr(VI) and/or melatonin intraperitoneal injection. We identified that the testicular damage caused by Cr(VI) persisted to Day 21 after administration and then started to be alleviated, with clear alleviation on Day 35. Pretreatment with melatonin evidently reduced Cr(VI)-induced testicular damage and accelerated spermatogenic restoration, generating an almost normal phenotype on Day 35. Melatonin pretreatment also retained the sperm quality at all time points investigated. Moreover, melatonin to some extent preserved the fertility of Cr(VI)-treated mice without apparent side effects. The findings shed light on the future clinical application of melatonin as a therapeutic agent for environmental heavy metal toxicant-induced male subfertility or infertility.

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来源期刊
Molecular human reproduction
Molecular human reproduction 生物-发育生物学
CiteScore
8.30
自引率
0.00%
发文量
37
审稿时长
6-12 weeks
期刊介绍: MHR publishes original research reports, commentaries and reviews on topics in the basic science of reproduction, including: reproductive tract physiology and pathology; gonad function and gametogenesis; fertilization; embryo development; implantation; and pregnancy and parturition. Irrespective of the study subject, research papers should have a mechanistic aspect.
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