亚洲鲈鱼两个三重基序基因的特征及其在病毒感染和微生物分子基序反应中的表达。

IF 1.5 4区 农林科学 Q3 FISHERIES Journal of aquatic animal health Pub Date : 2023-05-04 DOI:10.1002/aah.10187
Krishnapriya Raji Sathyan, Avinash Premraj, Sajeevan Thavarool Puthiyedathu
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引用次数: 1

摘要

目的:我们从亚洲鲈鱼Lates calcarifer中鉴定了两个三重基序(TRIM)基因,LcTRIM21和LcTRIM39,并用微生物病原体相关的分子模式检测了它们对实验性倍他野病毒感染和刺激的反应。方法:从亚洲鲈鱼中鉴定、克隆和测序编码LcTRIM21和LcTRIM39的基因。我们使用各种生物信息学工具分析了该序列,以确定蛋白质结构、定位并建立系统发育树。通过使用定量实时PCR,我们分析了LcTRIM21和LcTRIM39基因对倍他野病毒攻击的表达谱,以及与分子病原体相关的分子模式,如poly(I:C)和Zymosan A。还检测了这些基因在健康动物中的组织分布模式。结果:克隆了亚洲鲈鱼TRIM基因的同源物LcTRIM21和LcTRIM39,它们都编码547个氨基酸的蛋白质。LcTRIM21的等电点为6.32,分子量为62.11千道尔顿,而LcTRIM39的等电点将为5.57,分子量则为62.11千道尔顿。通过计算机蛋白质定位预测LcTRIM21和LcTRIM39同源物定位在细胞质中。从结构上讲,这两种蛋白质都含有一个N端非常有趣的新基因(RING)锌指结构域、B盒结构域、卷曲螺旋结构域和C端PRY/SPRY结构域。所检查的大多数组织和器官显示LcTRIM21和LcTRIM39的组成型表达。在poly(I:C)攻击或红斑石斑鱼神经坏死病毒感染后,LcTRIM21和LcTRIM39 mRNA表达显著上调,表明它们可能对鱼类病毒发挥关键的抗病毒作用。LcTRIM21和LcTRIM39的表达也通过给予葡聚糖Zymosan A而上调。结论:含有TRIM的基因是一种E3泛素连接酶,通过蛋白酶体介导的泛素化靶向病毒蛋白而表现出抗病毒活性。TRIM蛋白可用于发现抗病毒药物和对抗病毒性神经坏死等疾病的策略,这些疾病威胁着鲈鱼养殖。
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Characterization of two tripartite motif-containing genes from Asian Seabass Lates calcarifer and their expression in response to virus infection and microbial molecular motifs

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来源期刊
Journal of aquatic animal health
Journal of aquatic animal health 农林科学-兽医学
CiteScore
2.50
自引率
0.00%
发文量
25
审稿时长
>24 weeks
期刊介绍: The Journal of Aquatic Animal Health serves the international community of scientists and culturists concerned with the health of aquatic organisms. It carries research papers on the causes, effects, treatments, and prevention of diseases of marine and freshwater organisms, particularly fish and shellfish. In addition, it contains papers that describe biochemical and physiological investigations into fish health that relate to assessing the impacts of both environmental and pathogenic features.
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