Alternathera littoralis P.Beauv.未出现母婴不良反应。在小鼠怀孕期间进行治疗。

IF 2.3 4区 医学 Q3 ENVIRONMENTAL SCIENCES Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2023-08-18 Epub Date: 2023-06-21 DOI:10.1080/15287394.2023.2223624
Giovana Corbucci Danti Rezende, Renata Coelho Rodrigues Noronha, Hudman Cunha Ortiz, Luís Adriano Santos do Nascimento, Silvia Cordeiro das Neves, Yasmin Lany Ventura Said, Adauto Lima Cardoso, Vanessa Albuquerque de Mescouto, Marcelo Luiz Brandão Vilela, Valter Aragão do Nascimento, Henrique Rodrigues Scherer Coelho, Candida Aparecida Leite Kassuya, Taise Fonseca Pedroso, Marcos José Salvador, Rodrigo Juliano Oliveira
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引用次数: 1

摘要

Alternathera littoralis P.Beauv是一种原产于巴西的植物,具有多种有益活性,包括抗氧化、抗菌、抗真菌、抗原生动物、抗痛觉过敏和抗炎特性。本研究的目的是评估Alternathera littoralis(EEAl)的乙醇提取物对怀孕雌性小鼠的生殖结果、胚胎-胎儿发育和DNA完整性的影响。怀孕的瑞士雌性小鼠被随机分配到三个实验组(n = 10) :对照组给予1%吐温80(载体)、EEAl 100 mg/kg或EEAl 1000 mg/kg。在妊娠期至第18天通过灌胃给予治疗。在妊娠第16、17和18天,从尾静脉获得外周血样本用于DNA完整性分析(微核试验)。最后一次采集后,通过颈椎脱位对动物实施安乐死。采集母体器官和胎儿,称重,随后进行分析。通过测量植入物的数量、活胎儿和吸收来评估生殖结果参数。胚胎发育是通过孕龄体重的适当性以及外部、内脏和骨骼畸形的确定来确定的。数据表明,EEAl在两种剂量下都不会产生母体毒性,任何生殖结果参数(包括植入部位、活/死胎儿比例、胎儿生存能力、植入后损失、吸收和吸收率)都没有显著变化。然而,EEAl 1000组通过降低胎盘重量来减少胚胎-胎儿的发育。此外,EEAl 1000组的外部和骨骼畸形频率增加,这不能归因于提取物暴露,因为这些值在控制水平内。根据我们的研究结果,有证据表明,在我们研究中使用的浓度下的EEAl可能被认为在怀孕期间使用是安全的,这种植物的提取物显示出开发用于怀孕的植物药物的潜力。
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Absence of maternal-fetal adverse effects of Alternanthera littoralis P. Beauv. following treatment during pregnancy in mice.

Alternanthera littoralis P. Beauv is a plant native to Brazil that exhibits various beneficial activities including antioxidant, antibacterial, antifungal, antiprotozoal, anti-hyperalgesic, and anti-inflammatory properties. The aim of this study was to assess the impact of the ethanol extract of Alternanthera littoralis (EEAl) on reproductive outcomes, embryofetal development, and DNA integrity of pregnant female mice. Pregnant Swiss female mice were randomly assigned to three experimental groups (n = 10): controls were administered either 1% Tween 80 (vehicle), EEAl 100 mg/kg or EEAl 1000 mg/kg. Treatment was administered through gavage during the gestational period until day 18. On gestational days 16, 17, and 18, a peripheral blood sample from the tail vein was obtained for DNA integrity analysis (micronucleus test). After the last collection, animals were euthanized by cervical dislocation. Maternal organs and fetuses were collected, weighed, and subsequently analyzed. Reproductive outcome parameters were assessed by measurement of number of implants, live fetuses, and resorptions. Embryonic development was determined by adequacy of weight for gestational age as well as determination of external, visceral, and skeletal malformations. Data demonstrated that EEAl did not produce maternal toxicity at either dose associated with no marked alterations in any of the reproductive outcome parameters including implantation sites, live/dead fetuses ratio, fetal viability, post-implantation losses, resorptions, and resorption rate. However, EEAl 1000 group reduced embryofetal development by lowering placental weight. In addition, there was an increase in the frequency of external and skeletal malformations in the EEAl 1000 group, which could not be attributed to extract exposure as these values were within control levels. Based upon our findings, evidence indicates that the EEAl at the concentrations employed in our study may be considered safe for use during pregnancy and extracts of this plant show potential for development of phytomedicines to be used in pregnancy.

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来源期刊
CiteScore
5.20
自引率
19.20%
发文量
46
审稿时长
8-16 weeks
期刊介绍: The Journal of Toxicology and Environmental Health, Part A , Current Issues is an authoritative journal that features strictly refereed original research in the field of environmental sciences, public and occupational health, and toxicology.
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