AC125611.3通过募集DKC1稳定CTNNB1来促进癌症的进展。

IF 1.1 4区 医学 Q4 GASTROENTEROLOGY & HEPATOLOGY Arab Journal of Gastroenterology Pub Date : 2023-08-01 DOI:10.1016/j.ajg.2022.10.013
Hanqing Tang , Yuyu Dou , Yiliang Meng , Qinglan Lu , Lingling Liang
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引用次数: 0

摘要

背景和研究目的:先前的研究表明lncRNA影响癌症的进展。lncRNA AC125611.3(也称为RP11-161H23.5)在癌症中高表达,但很少研究;了解其调节可能为治疗结肠癌癌症提供新的见解。材料和方法:采用qRT-PCR对RNA进行定量。进行CCK-8和EdU测定以评估细胞增殖。Western印迹分析用于检测与细胞凋亡和EMT相关的蛋白质水平。分别进行伤口愈合试验和Transwell侵袭试验来评估细胞迁移和侵袭能力。荧光素酶报告基因测定法、RIP测定法和下拉测定法用于验证RNA-RNA和RNA-蛋白质的相互作用。结果:AC125611.3在结肠癌癌症细胞中高表达。AC125611.3耗竭抑制了细胞增殖、侵袭、迁移和EMT过程,同时增强了细胞凋亡。此外,AC125611.3通过调节连环蛋白β-1(CTNNB1)激活了癌症细胞中的Wnt信号通路。此外,AC125611.3招募先天性角化病1(DKC1)稳定CTNNB1。
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AC125611.3 promotes the progression of colon cancer by recruiting DKC1 to stabilize CTNNB1

Background and study aims

Previous studies have suggested that lncRNAs impact cancer progression. The lncRNA AC125611.3 (also referred to as RP11-161H23.5) is highly expressed in colon cancer but rarely studied; understanding its regulation may provide novel insights on treating colon cancer.

Materials and methods

qRT-PCR was performed to quantify RNAs. CCK-8 and EdU assays were performed to assess cell proliferation. Western blot analysis was used to detect levels of proteins related to cell apoptosis and EMT. Wound healing assay and Transwell invasion assay were conducted to evaluate cell migratory and invasive capabilities, respectively. Luciferase reporter assay, RIP assay, and pull-down assay were used to verify RNA-RNA and RNA-protein interactions.

Results

AC125611.3 was highly overexpressed in colon cancer cells. AC125611.3 depletion curbed cell proliferative, invasive, migratory, and EMT processes while enhancing apoptosis. Furthermore, AC125611.3 activated the Wnt signaling pathway in colon cancer cells by regulating catenin beta-1 (CTNNB1). Moreover, AC125611.3 recruited dyskeratosis congenita 1 (DKC1) to stabilize CTNNB1.

Conclusion

AC125611.3 recruits DKC1 to stabilize CTNNB1 and activate Wnt signaling, thereby promoting the progression of colon cancer.

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来源期刊
Arab Journal of Gastroenterology
Arab Journal of Gastroenterology Medicine-Gastroenterology
CiteScore
2.70
自引率
0.00%
发文量
52
期刊介绍: Arab Journal of Gastroenterology (AJG) publishes different studies related to the digestive system. It aims to be the foremost scientific peer reviewed journal encompassing diverse studies related to the digestive system and its disorders, and serving the Pan-Arab and wider community working on gastrointestinal disorders.
期刊最新文献
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