[脂肪-1基因转基因猪肉粉的蛋白质效率比]。

Shuhui Nie, Deqian Mao, Min Li, Weidong Li, Jiaxi Lu, Lichen Yang, Yichun Hu
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引用次数: 0

摘要

目的:评价含有脂肪-1基因的转基因猪肉粉(GM组)的蛋白质效率(PER),从而评价脂肪-1基因猪肉粉的营养评价价值是否发生变化。方法:60只断奶SD大鼠(60 ~ 80 g),按性别和体重随机分为酪蛋白组、亲本对照组和转基因组,每组20只,雌雄各占一半。三组大鼠分别饲喂相应的含10%蛋白质的配方饲料,饲喂28 d。每周记录各组的体重和食物摄入量。实验结束时采血,测定血液学及血液生化指标。计算食物利用率、脏器体重指数、PER和修正PER。结果:实验期间各组大鼠体重均呈稳定上升趋势。部分血液学、血液生化指标及脏器/体重指标差异有统计学意义。没有发现明显的生物学变化。转基因组的食物利用率高于酪蛋白组(P<0.05),与亲本对照组相当。添加脂肪-1基因的转基因猪肉粉和亲本白猪肉粉的PER均高于酪蛋白(P<0.05)。结论:脂肪-1基因转基因猪肉粉的PER与亲本白猪肉粉相当。
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[Protein efficiency ratio of genetically modified pork powder with fat-1 gene].

Objective: To evaluate the protein efficiency ratio(PER) of genetically modified pork powder with fat-1 gene(GM group), and thus evaluate whether the nutritional evaluation value of fat-1 gene pork powder has changed.

Methods: Sixty weaned SD rats(60-80 g) were randomly divided into casein group, parental control group and GM group according to sex and weight, 20 rats in each group, half of each sex. The rats in the three groups were fed with corresponding formulated feed containing 10% protein for 28 days. The body weight and food intake of each group were recorded weekly. Blood was collected at the end of the experiment to determine hematology and blood biochemical indexes. The food utilization rate, organ/body weigh indexes, PER and corrected PER were calculated.

Results: The weight of rats in all groups increased steadily during the experimental period. Statistically significant differences were found in some hematology and blood biochemical indexes and organ/body weigh indexes. No biologically significant changes were found. The food utilization rate of GM group was higher than that of casein group(P<0.05), which was equivalent to that in the parental control group. The PER of both genetically modified pork powder with fat-1 gene and parental white pork powder were higher than that of casein(P<0.05).

Conclusion: The PER of genetically modified pork powder with fat-1 gene was equal to that of its parental white pork powder.

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