Yanxin Jia, Yan Liu, Shuang Liu, Yaxin Chang, Longfei Xu, Haifang Li
{"title":"Wnt10b敲低可促进小鼠棕色脂肪组织中UCP1的表达。","authors":"Yanxin Jia, Yan Liu, Shuang Liu, Yaxin Chang, Longfei Xu, Haifang Li","doi":"10.1111/gtc.13064","DOIUrl":null,"url":null,"abstract":"<p>The effect of <i>Wnt10b</i> overexpression on adipose tissue development has been reported. However, the impact of <i>Wnt10b</i> knockdown on the function of brown adipose tissue (BAT) is yet largely unknown. Here, we used the CRISPR/Cas9 technique to generate <i>Wnt10b</i>-knockdown (<i>Wnt10b</i><sup>+/−</sup>) mice. We compared the development and thermogenic gene expression of interscapular BAT (iBAT) between <i>Wnt10b</i><sup>+/−</sup> and <i>Wnt10b</i><sup>+/+</sup> mice under a chow diet, high-fat diet (HFD), and cold exposure conditions. Moreover, the effect of <i>Wnt10b</i> knockdown on brown adipocyte function was tested via in vitro experiments. Results indicated that <i>Wnt10b</i> knockdown decreased the iBAT mass and the brown adipocyte size and enhanced thermogenic gene expression, including <i>UCP1</i>, under chow diet conditions. In addition, <i>Wnt10b</i><sup>+/−</sup> mice appeared to be able to maintain their body temperature better than the control in a cold environment, accompanied by higher UCP1 protein expression. Intriguingly, even under HFD conditions, <i>Wnt10b</i><sup>+/−</sup> mice still showed higher UCP1 expression, which was associated with an alleviated obesity phenotype. In vitro studies further evidenced the <i>Wnt10b</i> knockdown stimulation of UCP1 expression and suppression of the adipogenic program. This study indicates that <i>Wnt10b</i> knockdown enhances UCP1 expression and inhibits the adipogenic differentiation of brown adipocytes, providing a novel option for therapeutic interventions in adiposity.</p>","PeriodicalId":12742,"journal":{"name":"Genes to Cells","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Wnt10b knockdown promotes UCP1 expression in brown adipose tissue in mice\",\"authors\":\"Yanxin Jia, Yan Liu, Shuang Liu, Yaxin Chang, Longfei Xu, Haifang Li\",\"doi\":\"10.1111/gtc.13064\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The effect of <i>Wnt10b</i> overexpression on adipose tissue development has been reported. However, the impact of <i>Wnt10b</i> knockdown on the function of brown adipose tissue (BAT) is yet largely unknown. Here, we used the CRISPR/Cas9 technique to generate <i>Wnt10b</i>-knockdown (<i>Wnt10b</i><sup>+/−</sup>) mice. We compared the development and thermogenic gene expression of interscapular BAT (iBAT) between <i>Wnt10b</i><sup>+/−</sup> and <i>Wnt10b</i><sup>+/+</sup> mice under a chow diet, high-fat diet (HFD), and cold exposure conditions. Moreover, the effect of <i>Wnt10b</i> knockdown on brown adipocyte function was tested via in vitro experiments. Results indicated that <i>Wnt10b</i> knockdown decreased the iBAT mass and the brown adipocyte size and enhanced thermogenic gene expression, including <i>UCP1</i>, under chow diet conditions. In addition, <i>Wnt10b</i><sup>+/−</sup> mice appeared to be able to maintain their body temperature better than the control in a cold environment, accompanied by higher UCP1 protein expression. Intriguingly, even under HFD conditions, <i>Wnt10b</i><sup>+/−</sup> mice still showed higher UCP1 expression, which was associated with an alleviated obesity phenotype. In vitro studies further evidenced the <i>Wnt10b</i> knockdown stimulation of UCP1 expression and suppression of the adipogenic program. This study indicates that <i>Wnt10b</i> knockdown enhances UCP1 expression and inhibits the adipogenic differentiation of brown adipocytes, providing a novel option for therapeutic interventions in adiposity.</p>\",\"PeriodicalId\":12742,\"journal\":{\"name\":\"Genes to Cells\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2023-09-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genes to Cells\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/gtc.13064\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genes to Cells","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/gtc.13064","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
Wnt10b knockdown promotes UCP1 expression in brown adipose tissue in mice
The effect of Wnt10b overexpression on adipose tissue development has been reported. However, the impact of Wnt10b knockdown on the function of brown adipose tissue (BAT) is yet largely unknown. Here, we used the CRISPR/Cas9 technique to generate Wnt10b-knockdown (Wnt10b+/−) mice. We compared the development and thermogenic gene expression of interscapular BAT (iBAT) between Wnt10b+/− and Wnt10b+/+ mice under a chow diet, high-fat diet (HFD), and cold exposure conditions. Moreover, the effect of Wnt10b knockdown on brown adipocyte function was tested via in vitro experiments. Results indicated that Wnt10b knockdown decreased the iBAT mass and the brown adipocyte size and enhanced thermogenic gene expression, including UCP1, under chow diet conditions. In addition, Wnt10b+/− mice appeared to be able to maintain their body temperature better than the control in a cold environment, accompanied by higher UCP1 protein expression. Intriguingly, even under HFD conditions, Wnt10b+/− mice still showed higher UCP1 expression, which was associated with an alleviated obesity phenotype. In vitro studies further evidenced the Wnt10b knockdown stimulation of UCP1 expression and suppression of the adipogenic program. This study indicates that Wnt10b knockdown enhances UCP1 expression and inhibits the adipogenic differentiation of brown adipocytes, providing a novel option for therapeutic interventions in adiposity.
期刊介绍:
Genes to Cells provides an international forum for the publication of papers describing important aspects of molecular and cellular biology. The journal aims to present papers that provide conceptual advance in the relevant field. Particular emphasis will be placed on work aimed at understanding the basic mechanisms underlying biological events.